验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A2780 cells, COLO 320 cells, HEK-293 cells, MCF-7 cells |
| Positive IHC detected in | human liver cancer tissue, human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20985-1-AP targets DEPDC6/Deptor in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DEP domain containing 6 |
| 别名 | DEP domain containing 6, DEP.6, DEPDC6, DEPDC6/Deptor, Deptor |
| 计算分子量 | 46 kDa |
| 观测分子量 | 40-45 kDa |
| GenBank蛋白编号 | NM_022783 |
| 基因名称 | DEPDC6 |
| Gene ID (NCBI) | 64798 |
| RRID | AB_11182391 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TB45 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DEPDC6, also named as DEPTOR, was identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival. It negative regulator of the mTORC1 and mTORC2 signaling pathways. DEPDC6 inhibits the kinase activity of both complexes. The antibody is specific to DEPDC6.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DEPDC6/Deptor antibody 20985-1-AP | Download protocol |
| IHC protocol for DEPDC6/Deptor antibody 20985-1-AP | Download protocol |
| IF protocol for DEPDC6/Deptor antibody 20985-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Transl Med The PTH/PTHrP-SIK3 pathway affects skeletogenesis through altered mTOR signaling. | ||
Autophagy TNFAIP3-DEPTOR complex regulates inflammasome secretion through autophagy in ankylosing spondylitis monocytes. | ||
Cell Death Differ NUMB facilitates autophagy initiation through targeting SCFβ-TrCP2 complex. | ||
Oncotarget Inhibition of neddylation regulates dendritic cell functions via Deptor accumulation driven mTOR inactivation. | ||
Stem Cell Res Ther DEPTOR regulates osteogenic differentiation via inhibiting MEG3-mediated activation of BMP4 signaling and is involved in osteoporosis. | ||
