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DEPDC6 Polyclonal antibody

DEPDC6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21312-1-AP

Print datasheet

Synonyms

DEP domain containing 6, DEP.6, DEPDC6, Deptor

验证数据展示

  • WB analysis of HeLa using 21312-1-AP

    WB analysis of HeLa using 21312-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 21312-1-AP

    IHC staining of human thyroid cancer using 21312-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer slide using 21312-1-AP (DEPDC6 Antibody) at dilution of 1:50.

经过测试的应用

Positive WB detected inHeLa cells, COLO 320 cells, HT-1080 cells
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21312-1-AP is DEPDC6 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen DEPDC6 fusion protein Ag15959 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DEP domain containing 6
Synonyms DEP domain containing 6, DEP.6, DEPDC6, Deptor
Calculated Molecular Weight 409 aa, 46 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC024746
Gene Symbol DEPDC6
Gene ID (NCBI) 64798
RRIDAB_2878837
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TB45
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DEPTOR, also named as DEPDC6, is a negative regulator of the mTORC1 and mTORC2 signaling pathways. Through mTOR interactions, DEPTOR is implicated in pathways for cell growth and differentiation, as well as cell-cycle prolongation or termination. It inhibits the kinase activity of both complexes. The MW will migrate to 50-55 kDa with phospho- form.

实验方案

Product Specific Protocols
WB protocol for DEPDC6 antibody 21312-1-APDownload protocol
IHC protocol for DEPDC6 antibody 21312-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols