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验证数据展示

  • WB analysis of HepG2 using 26517-1-AP

    WB analysis of HepG2 using 26517-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26517-1-AP (DEPDC7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

DEPDC7 Polyclonal antibody

DEPDC7 Polyclonal Antibody for WB, ELISA
Cat No. 26517-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

TR2, Protein TR2/D15, DEP domain-containing protein 7, DEP domain containing 7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26517-1-AP targets DEPDC7 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 DEPDC7 fusion protein Ag24094 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DEP domain containing 7
别名 TR2, Protein TR2/D15, DEP domain-containing protein 7, DEP domain containing 7
观测分子量 55-60 kDa
GenBank蛋白编号BC030970
基因名称 DEPDC7
Gene ID (NCBI) 91614
RRIDAB_2880539
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96QD5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DEPDC7 (DEP domain-containing protein 7), also known as protein TR2/D15, is a 511 amino acid protein belonging to the DEPDC7 family. DEPDC7 contains one DEP domain and is highly expressed in liver, with lower levels in kidney. DEPDC7 exists as two isoforms produced by alternative splicing events. The gene encoding DEPDC7 maps to human chromosome 11p13 and mouse chromosome 2 E2. With approximately 135 million base pairs and 1,400 genes, chromosome 11 makes up around 4% of human genomic DNA and is considered a gene and disease association dense chromosome. DEPDC7 involves the regulation of small GTPase mediated signal transduction.

实验方案

Product Specific Protocols
WB protocol for DEPDC7 antibody 26517-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols