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DFNA5 Polyclonal antibody, PBS Only

DFNA5 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 31363-1-PBS

Print datasheet

Synonyms

Gasdermin-E, Gasdermin E, DFNA5/GSDME, GSDME, Gasdermin-E, C-terminal

验证数据展示

  • WB analysis using 31363-1-AP (same clone as 31363-1-PBS)

    WB analysis using 31363-1-AP (same clone as 31363-1-PBS)

    Untreated, and etoposide (60uM, 14h) treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 31363-1-AP (DFNA5/GSDME antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP (same clone as 31363-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP (same clone as 31363-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31363-1-AP (DFNA5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GSDME/DFNA5 antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP (same clone as 31363-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP (same clone as 31363-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31363-1-AP (DFNA5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GSDME/DFNA5 antibody included
  • IP experiment of SH-SY5Y using 31363-1-AP (same clone as 31363-1-PBS)

    IP experiment of SH-SY5Y using 31363-1-AP (same clone as 31363-1-PBS)

    IP result of anti-DFNA5/GSDME (IP:31363-1-AP, 4ug; Detection:31363-1-AP 1:800) with SH-SY5Y cells lysate 6790 ug. This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy GSDME/DFNA5 Ready-To-Use IHC Kit. GSDME/DFNA5 primary antibody included.

产品信息

31363-1-PBS targets DFNA5 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen DFNA5 fusion protein Ag35186 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deafness, autosomal dominant 5
Synonyms Gasdermin-E, Gasdermin E, DFNA5/GSDME, GSDME, Gasdermin-E, C-terminal
Calculated Molecular Weight 496 aa, 55 kDa
Observed Molecular Weight55 kDa, 35 kDa, 25 kDa
GenBank Accession NumberBC019689
Gene Symbol DFNA5
Gene ID (NCBI) 1687
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO60443
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. GSDME produced two GSDME fragments with MW of 35 kDa and 25 kDa.