验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue |
Planning an IHC experiment? We recommend our IHCeasy GSDME/DFNA5 Ready-To-Use IHC Kit. GSDME/DFNA5 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83454-6-RR targets DFNA5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | DFNA5 fusion protein Ag3746 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | deafness, autosomal dominant 5 |
| Synonyms | Gasdermin-E, Gasdermin E, DFNA5/GSDME, GSDME, DFNA 5 |
| Calculated Molecular Weight | 496 aa, 55 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC019689 |
| Gene Symbol | DFNA5 |
| Gene ID (NCBI) | 1687 |
| RRID | AB_3671090 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | O60443 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. GSDME produced two GSDME fragments with MW of 35 kDa and 25 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DFNA5 antibody 83454-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |