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DFNA5/GSDME Polyclonal antibody

DFNA5/GSDME Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30696-1-AP

Print datasheet

Synonyms

2310037D07Rik, 4932441K13Rik, Dfna5, Dfna5h, EG14210, Fin15, Gasdermin-E

验证数据展示

  • WB analysis using 30696-1-AP

    WB analysis using 30696-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30696-1-AP (DFNA5/GSDME antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJ774A.1 cells, mouse brain tissue, rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30696-1-AP targets DFNA5/GSDME in WB, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity mouse
Immunogen DFNA5/GSDME fusion protein Ag33622 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deafness, autosomal dominant 5 (human)
Synonyms 2310037D07Rik, 4932441K13Rik, Dfna5, Dfna5h, EG14210, Fin15, Gasdermin-E
Calculated Molecular Weight57 kDa
Observed Molecular Weight57 kDa
GenBank Accession NumberBC132303
Gene Symbol DFNA5/GSDME
Gene ID (NCBI) 54722
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Z2D3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain.

实验方案

Product Specific Protocols
WB protocol for DFNA5/GSDME antibody 30696-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols