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验证数据展示

  • WB analysis using 31363-1-AP

    WB analysis using 31363-1-AP

    Untreated, and etoposide (60uM, 14h) treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 31363-1-AP (DFNA5/GSDME antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31363-1-AP (DFNA5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GSDME/DFNA5 antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31363-1-AP (DFNA5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GSDME/DFNA5 antibody included
  • IP experiment of SH-SY5Y using 31363-1-AP

    IP experiment of SH-SY5Y using 31363-1-AP

    IP result of anti-DFNA5/GSDME (IP:31363-1-AP, 4ug; Detection:31363-1-AP 1:800) with SH-SY5Y cells lysate 6790 ug.

  • IF Staining of SH-SY5Y using 31363-1-AP

    IF Staining of SH-SY5Y using 31363-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DFNA5 antibody (31363-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DFNA5 Polyclonal antibody

DFNA5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 31363-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Gasdermin-E, Gasdermin E, DFNA5/GSDME, GSDME, Gasdermin-E, C-terminal

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, A549 cells, mouse brain tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy GSDME/DFNA5 Ready-To-Use IHC Kit. GSDME/DFNA5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31363-1-AP targets DFNA5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen DFNA5 fusion protein Ag35186 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deafness, autosomal dominant 5
Synonyms Gasdermin-E, Gasdermin E, DFNA5/GSDME, GSDME, Gasdermin-E, C-terminal
Calculated Molecular Weight 496 aa, 55 kDa
Observed Molecular Weight55 kDa, 35 kDa, 25 kDa
GenBank Accession NumberBC019689
Gene Symbol DFNA5
Gene ID (NCBI) 1687
RRIDAB_3669957
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO60443
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. GSDME produced two GSDME fragments with MW of 35 kDa and 25 kDa.

实验方案

Product Specific Protocols
WB protocol for DFNA5 antibody 31363-1-APDownload protocol
IHC protocol for DFNA5 antibody 31363-1-APDownload protocol
IF protocol for DFNA5 antibody 31363-1-APDownload protocol
IP protocol for DFNA5 antibody 31363-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols