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DFNA5/GSDME Polyclonal antibody

DFNA5/GSDME Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 31363-1-AP

Print datasheet

Synonyms

deafness, autosomal dominant 5, DFNA5, Gasdermin E, Gasdermin-E, GSDME, ICERE 1, ICERE1, N-terminal GSDME

验证数据展示

  • WB analysis using 31363-1-AP

    WB analysis using 31363-1-AP

    Untreated, and etoposide (60uM, 14h) treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 31363-1-AP (DFNA5/GSDME antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy GSDME/DFNA5 Ready-To-Use IHC Kit. GSDME/DFNA5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31363-1-AP targets DFNA5/GSDME in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen DFNA5/GSDME fusion protein Ag35186 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deafness, autosomal dominant 5
Synonyms deafness, autosomal dominant 5, DFNA5, Gasdermin E, Gasdermin-E, GSDME, ICERE 1, ICERE1, N-terminal GSDME
Calculated Molecular Weight 496 aa, 55 kDa
Observed Molecular Weight55 kDa, 35 kDa, 25 kDa
GenBank Accession NumberBC019689
Gene Symbol DFNA5
Gene ID (NCBI) 1687
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO60443
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. GSDME produced two GSDME fragments with MW of 35 kDa and 25 kDa.

实验方案

Product Specific Protocols
WB protocol for DFNA5/GSDME antibody 31363-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols