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验证数据展示

  • WB analysis using 31363-1-AP

    WB analysis using 31363-1-AP

    Untreated, and etoposide (60uM, 14h) treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 31363-1-AP (DFNA5/GSDME antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31363-1-AP (DFNA5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GSDME/DFNA5 antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31363-1-AP (DFNA5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GSDME/DFNA5 antibody included
  • IP experiment of SH-SY5Y using 31363-1-AP

    IP experiment of SH-SY5Y using 31363-1-AP

    IP result of anti-DFNA5/GSDME (IP:31363-1-AP, 4ug; Detection:31363-1-AP 1:800) with SH-SY5Y cells lysate 6790 ug.

  • IF Staining of SH-SY5Y using 31363-1-AP

    IF Staining of SH-SY5Y using 31363-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DFNA5 antibody (31363-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DFNA5/GSDME Polyclonal antibody

DFNA5/GSDME Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 31363-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

DFNA5, Gasdermin E, Gasdermin-E, GSDME, deafness, autosomal dominant 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, A549 cells, mouse brain tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy GSDME/DFNA5 Ready-To-Use IHC Kit. GSDME/DFNA5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31363-1-AP targets DFNA5/GSDME in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 DFNA5/GSDME fusion protein Ag35186 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 deafness, autosomal dominant 5
别名 DFNA5, Gasdermin E, Gasdermin-E, GSDME, deafness, autosomal dominant 5
计算分子量 496 aa, 55 kDa
观测分子量55 kDa, 35 kDa, 25 kDa
GenBank蛋白编号BC019689
基因名称 DFNA5
Gene ID (NCBI) 1687
RRIDAB_3669957
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO60443
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. GSDME produced two GSDME fragments with MW of 35 kDa and 25 kDa.

实验方案

Product Specific Protocols
WB protocol for DFNA5/GSDME antibody 31363-1-APDownload protocol
IHC protocol for DFNA5/GSDME antibody 31363-1-APDownload protocol
IF protocol for DFNA5/GSDME antibody 31363-1-APDownload protocol
IP protocol for DFNA5/GSDME antibody 31363-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols