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DGKB Polyclonal antibody

DGKB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24320-1-AP

Print datasheet

Synonyms

90 kDa diacylglycerol kinase, DAG kinase beta, DAGK2, DGK, DGK BETA, DGKB, Diacylglycerol kinase beta, Diglyceride kinase beta, KIAA0718

验证数据展示

  • WB analysis of HeLa using 24320-1-AP

    WB analysis of HeLa using 24320-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24320-1-AP (DGKB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 24320-1-AP

    IHC staining of human breast cancer using 24320-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24320-1-AP (DGKB Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 24320-1-AP

    IHC staining of human breast cancer using 24320-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24320-1-AP (DGKB Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24320-1-AP is DGKB Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen DGKB fusion protein Ag19809 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name diacylglycerol kinase, beta 90kDa
Synonyms 90 kDa diacylglycerol kinase, DAG kinase beta, DAGK2, DGK, DGK BETA, DGKB, Diacylglycerol kinase beta, Diglyceride kinase beta, KIAA0718
Calculated Molecular Weight 804 aa, 91 kDa
Observed Molecular Weight 91 kDa
GenBank Accession NumberBC105005
Gene Symbol DGKB
Gene ID (NCBI) 1607
RRIDAB_2879492
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6T7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DGKB(Diacylglycerol kinase beta) is also named as DAGK2, KIAA0718 , 90 kDa diacylglycerol kinase and belongs to the eukaryotic diacetylglycerol kinase family. It is a key enzyme in lipid metabolism that functions to reintroduce diacylglycerol formed from the hydrolysis of phospholipids into the biosynthetic pathway. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 87 kDa and 91 kDa.

实验方案

Product Specific Protocols
WB protocol for DGKB antibody 24320-1-APDownload protocol
IHC protocol for DGKB antibody 24320-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols