• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HeLa using 24320-1-AP

    WB analysis of HeLa using 24320-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24320-1-AP (DGKB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 24320-1-AP

    IHC staining of human breast cancer using 24320-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24320-1-AP (DGKB Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 24320-1-AP

    IHC staining of human breast cancer using 24320-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24320-1-AP (DGKB Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DGKB Polyclonal antibody

DGKB Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24320-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

90 kDa diacylglycerol kinase, DAG kinase beta, DAGK2, DGK, DGK BETA, DGKB, Diacylglycerol kinase beta, Diglyceride kinase beta, KIAA0718

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24320-1-AP targets DGKB in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 DGKB fusion protein Ag19809 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 diacylglycerol kinase, beta 90kDa
别名 90 kDa diacylglycerol kinase, DAG kinase beta, DAGK2, DGK, DGK BETA, DGKB, Diacylglycerol kinase beta, Diglyceride kinase beta, KIAA0718
计算分子量 804 aa, 91 kDa
观测分子量 91 kDa
GenBank蛋白编号BC105005
基因名称 DGKB
Gene ID (NCBI) 1607
RRIDAB_2879492
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6T7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DGKB(Diacylglycerol kinase beta) is also named as DAGK2, KIAA0718 , 90 kDa diacylglycerol kinase and belongs to the eukaryotic diacetylglycerol kinase family. It is a key enzyme in lipid metabolism that functions to reintroduce diacylglycerol formed from the hydrolysis of phospholipids into the biosynthetic pathway. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 87 kDa and 91 kDa.

实验方案

Product Specific Protocols
WB protocol for DGKB antibody 24320-1-APDownload protocol
IHC protocol for DGKB antibody 24320-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols