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  • Featured Product
  • KD/KO Validated

DGKQ Polyclonal antibody

DGKQ Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 17885-1-AP

Print datasheet

Synonyms

DGK-theta, DGKtheta, DGK theta, DAGK7, DAGK4

验证数据展示

  • WB analysis of mouse brain using 17885-1-AP

    WB analysis of mouse brain using 17885-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17885-1-AP (DGKQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis using 17885-1-AP

    WB analysis using 17885-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 17885-1-AP (DGKQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 17885-1-AP

    IHC staining of human colon cancer using 17885-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17885-1-AP (DGKQ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DGKQ antibody included
  • IHC staining of human colon cancer using 17885-1-AP

    IHC staining of human colon cancer using 17885-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17885-1-AP (DGKQ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DGKQ antibody included
  • IF Staining of mouse small intestine using 17885-1-AP

    IF Staining of mouse small intestine using 17885-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using DGKQ antibody (17885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse kidney using 17885-1-AP

    IF Staining of mouse kidney using 17885-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using DGKQ antibody (17885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, human placenta tissue, THP-1 cells
Positive IHC detected inhuman colon cancer tissue, human cervix tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue, mouse kidney tissue
Planning an IHC experiment? We recommend our IHCeasy DGKQ Ready-To-Use IHC Kit. DGKQ primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17885-1-AP targets DGKQ in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DGKQ fusion protein Ag11938 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name diacylglycerol kinase, theta 110kDa
Synonyms DGK-theta, DGKtheta, DGK theta, DAGK7, DAGK4
Calculated Molecular Weight 942 aa, 101 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC063801
Gene Symbol DGKQ
Gene ID (NCBI) 1609
RRIDAB_2091305
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52824
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DGKQ antibody 17885-1-APDownload protocol
IHC protocol for DGKQ antibody 17885-1-APDownload protocol
IF protocol for DGKQ antibody 17885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Med Rep

Establishment of a novel hepatic steatosis cell model by Cas9/sgRNA-mediated DGKθ gene knockout.

Authors - Jingjing Zhang
  • KO Validated
View Article
WB

J Sci Food Agric

Neonatal resveratrol administration promotes skeletal muscle growth and insulin sensitivity in intrauterine growth-retarded suckling piglets associated with activation of FGF21-AMPKα pathway

Authors - Guangyi Bai
View Article