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验证数据展示

  • WB analysis using 32124-1-AP

    WB analysis using 32124-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32124-1-AP (DGKZ antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 32124-1-AP

    IF Staining of A431 using 32124-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DGKZ antibody (32124-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DGKZ Polyclonal antibody

DGKZ Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32124-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Diacylglycerol kinase zeta, DGK-zeta, DGK ZETA, DAGK6, DAGK5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32124-1-AP targets DGKZ in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DGKZ fusion protein Ag37336 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 diacylglycerol kinase, zeta 104kDa
别名 Diacylglycerol kinase zeta, DGK-zeta, DGK ZETA, DAGK6, DAGK5
计算分子量104kDa，928aa
观测分子量 104-124 kDa
GenBank蛋白编号NM_001199267.1
基因名称 DGKZ
Gene ID (NCBI) 8525
RRIDAB_3670200
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ13574
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Diacylglycerol kinase zeta (DGKZ), also known as DAGK5, DAGK6, DGK-ZETA, hDGKzeta, belongs to the diacylglycerol kinase (DGK) family which catalyzes diacylglycerol (DAG) into phosphatidic acid (PA). DGKZ is a novel promoter of metastasis and that it could be a potential prognostic indicator in patients with triple-negative breast cancer by suppressing lipid raft-dependent endocytosis of TGFbetaR2(PMID: 35115500). Downregulation of DGKZ suppresses tumorigenesis and progression of cervical cancer by facilitating cell apoptosis and cell cycle arrest(PMID: 33926342).

实验方案

Product Specific Protocols
WB protocol for DGKZ antibody 32124-1-APDownload protocol
IF protocol for DGKZ antibody 32124-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols