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dGK Polyclonal antibody

dGK Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16459-1-AP

Print datasheet

Synonyms

DGUOK, EC:2.7.1.76, EC:2.7.1.113, Deoxyguanosine kinase, Deoxyadenosine kinase, mitochondrial

验证数据展示

  • IHC staining of human skin cancer using 16459-1-AP

    IHC staining of human skin cancer using 16459-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 16459-1-AP (dGK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse kidney using 16459-1-AP

    IP experiment of mouse kidney using 16459-1-AP

    IP result of anti-dGK (IP:16459-1-AP, 3ug; Detection:16459-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

经过测试的应用

Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16459-1-AP targets dGK in IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen dGK fusion protein Ag9501 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deoxyguanosine kinase
Synonyms DGUOK, EC:2.7.1.76, EC:2.7.1.113, Deoxyguanosine kinase, Deoxyadenosine kinase, mitochondrial
Calculated Molecular Weight 277 aa, 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC015757
Gene Symbol DGUOK
Gene ID (NCBI) 1716
RRIDAB_2878260
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16854
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for dGK antibody 16459-1-APDownload protocol
IP protocol for dGK antibody 16459-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols