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验证数据展示

  • WB analysis using 15194-1-AP (same clone as 15194-1-PBS)

    WB analysis using 15194-1-AP (same clone as 15194-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15194-1-AP (DHFR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 15194-1-AP (same clone as 15194-1-PBS)

    WB analysis of HeLa using 15194-1-AP (same clone as 15194-1-PBS)

    WB result of DHFR antibody (15194-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHFR transfected HeLa cells. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

  • IP experiment of Raji using 15194-1-AP (same clone as 15194-1-PBS)

    IP experiment of Raji using 15194-1-AP (same clone as 15194-1-PBS)

    IP result of anti-DHFR (IP:15194-1-AP, 3ug; Detection:15194-1-AP 1:500) with Raji cells lysate 2000ug. This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 15194-1-AP (same clone as 15194-1-PBS)

    IHC staining of human liver cancer using 15194-1-AP (same clone as 15194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 15194-1-AP (same clone as 15194-1-PBS)

    IHC staining of human liver cancer using 15194-1-AP (same clone as 15194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15194-1-AP (DHFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 15194-1-AP (same clone as 15194-1-PBS)

    IF Staining of HeLa using 15194-1-AP (same clone as 15194-1-PBS)

    Immunofluorescent analysis of HeLa cells using 15194-1-AP (DHFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15194-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

DHFR Polyclonal antibody, PBS Only

DHFR Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 15194-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

15194-1-PBS targets DHFR in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DHFR fusion protein Ag7331 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dihydrofolate reductase
别名
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC000192
基因名称 DHFR
Gene ID (NCBI) 1719
RRIDAB_2091587
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP00374
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).