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DHPS Polyclonal antibody

DHPS Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11184-1-AP

Print datasheet

Synonyms

MIG13, EC:2.5.1.46, DS, DHS

验证数据展示

  • WB analysis of HeLa using 11184-1-AP

    WB analysis of HeLa using 11184-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11184-1-AP (DHPS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of NIH/3T3 using 11184-1-AP

    IP experiment of NIH/3T3 using 11184-1-AP

    IP result of anti-DHPS (IP:11184-1-AP, 4ug; Detection:11184-1-AP 1:300) with NIH/3T3 cells lysate 3200 ug.

  • IF Staining of HeLa using 11184-1-AP

    IF Staining of HeLa using 11184-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DHPS antibody (11184-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inNIH/3T3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

11184-1-AP targets DHPS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DHPS fusion protein Ag1646 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deoxyhypusine synthase
Synonyms MIG13, EC:2.5.1.46, DS, DHS
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC014016
Gene Symbol DHPS
Gene ID (NCBI) 1725
RRIDAB_2877751
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49366
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHPS(Deoxyhypusine synthase) catalyzes the conversion of lysine to deoxyhypusine residue on the eukaryotic initiation factor 5A (eIF-5A) precursor using spermidine as the substrate. It belongs to the deoxyhypusine synthase family. This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for DHPS antibody 11184-1-APDownload protocol
IF protocol for DHPS antibody 11184-1-APDownload protocol
IP protocol for DHPS antibody 11184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

BMC Med

Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke.

Authors - Takaki Hiwasa
View Article