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DHRS1 Polyclonal antibody

DHRS1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16275-1-AP

Print datasheet

Synonyms

Short chain dehydrogenase/reductase family 19C member 1, SDR19C1, Protein SDR19C1, EC:1.1.1.-

验证数据展示

  • IHC staining of human kidney using 16275-1-AP

    IHC staining of human kidney using 16275-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16275-1-AP (DHRS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 16275-1-AP

    WB analysis using 16275-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16275-1-AP (DHRS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 16275-1-AP

    WB analysis using 16275-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16275-1-AP (DHRS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 16275-1-AP

    IF Staining of A431 using 16275-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DHRS1 antibody (16275-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inCOLO 320 cells, human liver tissue, mouse colon tissue, mouse testis tissue
Positive IHC detected inhuman kidney tissue, human skin tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16275-1-AP targets DHRS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DHRS1 fusion protein Ag9351 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dehydrogenase/reductase (SDR family) member 1
Synonyms Short chain dehydrogenase/reductase family 19C member 1, SDR19C1, Protein SDR19C1, EC:1.1.1.-
Calculated Molecular Weight 313 aa, 34 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC014057
Gene Symbol DHRS1
Gene ID (NCBI) 115817
RRIDAB_2292847
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96LJ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHRS1(Dehydrogenase/reductase SDR family member 1) is also named as LJ25430, MGC20204, DKFZp586I0523, SDR19C1, FLJ14250 and belongs to the short-chain dehydrogenases/reductases (SDR) family. It is involved in the metabolism of a large variety of compounds, including steroid hormones, prostaglandins, retinoids, lipids and xenobiotics.Northern blot analysis detected DHRS1express in heart highest and in liver lowest.

实验方案

Product Specific Protocols
WB protocol for DHRS1 antibody 16275-1-APDownload protocol
IHC protocol for DHRS1 antibody 16275-1-APDownload protocol
IF protocol for DHRS1 antibody 16275-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols