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DHRS11 Polyclonal antibody

DHRS11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20172-1-AP

Print datasheet

Synonyms

ARPG836, DHRS11, SDR24C1

验证数据展示

  • WB analysis using 20172-1-AP

    WB analysis using 20172-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20172-1-AP (DHRS11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 20172-1-AP

    IHC staining of mouse small intestine using 20172-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20172-1-AP (DHRS11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse small intestine using 20172-1-AP

    IHC staining of mouse small intestine using 20172-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20172-1-AP (DHRS11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse kidney tissue, COLO 320 cells, mouse liver tissue
Positive IHC detected inmouse small intestine tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20172-1-AP targets DHRS11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DHRS11 fusion protein Ag14005 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dehydrogenase/reductase (SDR family) member 11
Synonyms ARPG836, DHRS11, SDR24C1
Calculated Molecular Weight 260 aa, 28 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC002731
Gene Symbol DHRS11
Gene ID (NCBI) 79154
RRIDAB_10733521
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UWP2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DHRS11 antibody 20172-1-APDownload protocol
IHC protocol for DHRS11 antibody 20172-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

Pericytes recruited by CCL28 promote vascular normalization after anti-angiogenesis therapy through RA/RXRA/ANGPT1 pathway in lung adenocarcinoma

Authors - Ying Chen
View Article