验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, mouse liver tissue, rat liver tissue, mouse kidney tissue |
| Positive IP detected in | A375 cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15393-1-AP targets DHRS3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, insect |
| 免疫原 | DHRS3 fusion protein Ag7589 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dehydrogenase/reductase (SDR family) member 3 |
| 别名 | retSDR1, Retinol dehydrogenase 17, Retinal short-chain dehydrogenase/reductase 1, RDH17, EC:1.1.1.300 |
| 计算分子量 | 34 kDa |
| 观测分子量 | 30-34 kDa |
| GenBank蛋白编号 | BC002730 |
| 基因名称 | DHRS3 |
| Gene ID (NCBI) | 9249 |
| RRID | AB_2091855 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75911 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DHRS3(Short-chain dehydrogenase/reductase 3) is also named as retSDR1,DD83.1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. It catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH. It is detected high levels of expression in fetal kidney, liver, and lung and in adult heart, placenta, lung, liver, kidney, pancreas, thyroid, testis, stomach, trachea, spinal cord and lower levels in skeletal muscle, intestine, and lymph node. retSDR1 is barely detectable in adrenals, brain, thymus, and hematopoietic tissues(PMID:11861404). retSDR1 may play a more general role in retinol metabolism since its expression was observed in many fetal and adult tissues(PMID:20543567).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DHRS3 antibody 15393-1-AP | Download protocol |
| IHC protocol for DHRS3 antibody 15393-1-AP | Download protocol |
| IF protocol for DHRS3 antibody 15393-1-AP | Download protocol |
| IP protocol for DHRS3 antibody 15393-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Endocrinol Metab Elevated periimplantation uterine natural killer cell density in human endometrium is associated with impaired corticosteroid signaling in decidualizing stromal cells. | ||
FASEB J The retinaldehyde reductase DHRS3 is essential for preventing the formation of excess retinoic acid during embryonic development. | ||
J Biol Chem Retinol dehydrogenase 11 is essential for the maintenance of retinol homeostasis in liver and testis in mice.
| ||
FASEB J Liver regeneration and ethanol detoxification: A new link in YAP regulation of ALDH1A1 during alcohol-related hepatocyte damage. | ||
Mol Endocrinol Induction of 11β-HSD 1 and activation of distinct mineralocorticoid receptor- and glucocorticoid receptor-dependent gene networks in decidualizing human endometrial stromal cells. | ||
J Biol Chem Retinol dehydrogenase 10 but not retinol/sterol dehydrogenase(s) regulates the expression of retinoic acid-responsive genes in human transgenic skin raft culture. |
