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验证数据展示

  • WB analysis using 83581-3-RR

    WB analysis using 83581-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83581-3-RR (DHRS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 83581-3-RR

    IF Staining of HepG2 using 83581-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DHRS3 antibody (83581-3-RR, Clone: 240545D8 ) at dilution of 1:100 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • FC experiment of HepG2 using 83581-3-RR

    FC experiment of HepG2 using 83581-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug DHRS3 Recombinant antibody (83581-3-RR, Clone:240545D8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83581-3-RR

    Affinity and Kinetic Characterization of 83581-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83548-3-RR against Human GHITM were performed. The affinity constant is 2.41 nM. Biolayer interferometry (BLl) kinetic assays of 83581-3-RR against Human DHRS3 were performed. The affinity constant is 0.109 nM.

DHRS3 Recombinant antibody

DHRS3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83581-3-RR
CloneNo. 240545D8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Retinol dehydrogenase 17, Retinal short-chain dehydrogenase/reductase 1, RDH17, EC:1.1.1.300, DD83.1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, mouse liver tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DHRS3 Ready-To-Use IHC Kit. DHRS3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83581-3-RR targets DHRS3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DHRS3 fusion protein Ag7589 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name dehydrogenase/reductase (SDR family) member 3
Synonyms Retinol dehydrogenase 17, Retinal short-chain dehydrogenase/reductase 1, RDH17, EC:1.1.1.300, DD83.1
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberBC002730
Gene Symbol DHRS3
Gene ID (NCBI) 9249
RRIDAB_3671195
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75911
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHRS3(Short-chain dehydrogenase/reductase 3) is also named as retSDR1,DD83.1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. It catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH. It is detected high levels of expression in fetal kidney, liver, and lung and in adult heart, placenta, lung, liver, kidney, pancreas, thyroid, testis, stomach, trachea, spinal cord and lower levels in skeletal muscle, intestine, and lymph node. retSDR1 is barely detectable in adrenals, brain, thymus, and hematopoietic tissues(PMID:11861404). retSDR1 may play a more general role in retinol metabolism since its expression was observed in many fetal and adult tissues(PMID:20543567).

实验方案

Product Specific Protocols
WB protocol for DHRS3 antibody 83581-3-RRDownload protocol
IF protocol for DHRS3 antibody 83581-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols