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DHRS4 Polyclonal antibody

DHRS4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15279-1-AP

Print datasheet

Synonyms

CR, DHRS4, DHRS4L2, humNRDR, NRDR, PHCR, PSCD, SCAD SRL, SDR SRL, SDR25C1

验证数据展示

  • WB analysis of rat liver using 15279-1-AP

    WB analysis of rat liver using 15279-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis using 15279-1-AP

    WB analysis using 15279-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15279-1-AP (DHRS4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 15279-1-AP

    IHC staining of human cervical cancer using 15279-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15279-1-AP (DHRS4 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 15279-1-AP

    IHC staining of human cervical cancer using 15279-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15279-1-AP (DHRS4 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse liver using 15279-1-AP

    IP experiment of mouse liver using 15279-1-AP

    IP result of anti-DHRS4 (IP:15279-1-AP, 4ug; Detection:15279-1-AP 1:400) with mouse liver tissue lysate 4000ug.

经过测试的应用

Positive WB detected inrat liver tissue, mouse liver tissue, HeLa cells, HepG2 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman cervical cancer tissue, human cervix tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15279-1-AP targets DHRS4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen DHRS4 fusion protein Ag7398 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dehydrogenase/reductase (SDR family) member 4
Synonyms CR, DHRS4, DHRS4L2, humNRDR, NRDR, PHCR, PSCD, SCAD SRL, SDR SRL, SDR25C1
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC003019
Gene Symbol DHRS4
Gene ID (NCBI) 10901
RRIDAB_2292856
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BTZ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHRS4(Dehydrogenase/reductase SDR family member 4) is also named as PHCR , NRDR ,PSCD , SCAD-SRL, and belongs to the short-chain dehydrogenases/reductases (SDR) family. It can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Human DHRS4 is a tetramer composed of 27 kDa subunits, which is inactivated at low temperature without dissociation into subunits(PMID:18571493).

实验方案

Product Specific Protocols
WB protocol for DHRS4 antibody 15279-1-APDownload protocol
IHC protocol for DHRS4 antibody 15279-1-APDownload protocol
IP protocol for DHRS4 antibody 15279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Immunol

Identification of Molecular Correlations Between DHRS4 and Progressive Neurodegeneration in Amyotrophic Lateral Sclerosis By Gene Co-Expression Network Analysis.

Authors - Shu Li
View Article