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Species Reactivity
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Conjugate
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DHRS9 Polyclonal antibody, PBS Only

DHRS9 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14560-1-PBS

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Synonyms

验证数据展示

  • IF Staining of A549 using 14560-1-AP (same clone as 14560-1-PBS)

    IF Staining of A549 using 14560-1-AP (same clone as 14560-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using DHRS9 antibody (14560-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14560-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 14560-1-AP (same clone as 14560-1-PBS)

    IHC staining of human lung cancer using 14560-1-AP (same clone as 14560-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14560-1-AP (DHRS9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14560-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 14560-1-AP (same clone as 14560-1-PBS)

    IHC staining of human lung cancer using 14560-1-AP (same clone as 14560-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14560-1-AP (DHRS9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14560-1-PBS in a different storage buffer formulation.

  • WB analysis using 14560-1-AP (same clone as 14560-1-PBS)

    WB analysis using 14560-1-AP (same clone as 14560-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14560-1-AP (DHRS9 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14560-1-PBS in a different storage buffer formulation.

产品信息

14560-1-PBS targets DHRS9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DHRS9 fusion protein Ag6116 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dehydrogenase/reductase (SDR family) member 9
Synonyms
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC058883
Gene Symbol DHRS9
Gene ID (NCBI) 10170
RRIDAB_2277277
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BPW9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Dehydrogenase/reductase 9(DHRS9), is a member of the short-chain dehydrogenases/reductases (SDR) family. DHRS9 has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. DHRS9 has 4 isoforms with molecular weight of 19, 30, 35, 42 kDa, and demonstrates oxidoreductase activity toward hydroxysteroids. DHRS9 is able to convert 3-alpha-tetrahydroprogesterone to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone in the cytoplasm, and may additionally function as a transcriptional repressor in the nucleus.