验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, NIH/3T3 cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
12265-1-AP targets DHX15 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, zebrafish |
| 免疫原 | DHX15 fusion protein Ag2895 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DEAH (Asp-Glu-Ala-His) box polypeptide 15 |
| 别名 | ATP dependent RNA helicase #46, DBP1, DDX15, DEAH box protein 15, DHX15, HRH2, PRP43, PRPF43, PrPp43p |
| 计算分子量 | 795 aa, 91 kDa |
| 观测分子量 | 91 kDa |
| GenBank蛋白编号 | BC035974 |
| 基因名称 | DHX15 |
| Gene ID (NCBI) | 1665 |
| RRID | AB_908769 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43143 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DHX15, also named as DBP1 and DDX15, is a DExD/H-box protein that has been identified in spliceosome, through its carboxyl (C)-terminal G-patch domain and stimulates the helicase activity of DHX15. DHX15 is a Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. The MW of DHX15 is about 90-95kd.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DHX15 antibody 12265-1-AP | Download protocol |
| IHC protocol for DHX15 antibody 12265-1-AP | Download protocol |
| IP protocol for DHX15 antibody 12265-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Fusobacterium nucleatum promotes tumor progression in KRAS p.G12D-mutant colorectal cancer by binding to DHX15 | ||
Cancer Sci Dhx15 regulates zebrafish definitive hematopoiesis through the unfolded protein response pathway. | ||
FEBS Lett Tumor suppressor RBM5 directly interacts with the DExD/H-box protein DHX15 and stimulates its helicase activity. | ||
PLoS Pathog Downregulation of CPSF6 Leads to Global mRNA 3' UTR shortening and enhanced antiviral immune responses |
