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DHX15 Polyclonal antibody

DHX15 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 12265-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

ATP dependent RNA helicase #46, DBP1, DDX15, DEAH box protein 15, DHX15, HRH2, PRP43, PRPF43, PrPp43p

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12265-1-AP targets DHX15 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, zebrafish
Immunogen DHX15 fusion protein Ag2895 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 15
Synonyms ATP dependent RNA helicase #46, DBP1, DDX15, DEAH box protein 15, DHX15, HRH2, PRP43, PRPF43, PrPp43p
Calculated Molecular Weight 795 aa, 91 kDa
Observed Molecular Weight 91 kDa
GenBank Accession NumberBC035974
Gene Symbol DHX15
Gene ID (NCBI) 1665
RRIDAB_908769
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43143
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHX15, also named as DBP1 and DDX15, is a DExD/H-box protein that has been identified in spliceosome, through its carboxyl (C)-terminal G-patch domain and stimulates the helicase activity of DHX15. DHX15 is a Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. The MW of DHX15 is about 90-95kd.

实验方案

Product Specific Protocols
WB protocol for DHX15 antibody 12265-1-APDownload protocol
IHC protocol for DHX15 antibody 12265-1-APDownload protocol
IP protocol for DHX15 antibody 12265-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Nat Commun

Fusobacterium nucleatum promotes tumor progression in KRAS p.G12D-mutant colorectal cancer by binding to DHX15

Authors - Huiyuan Zhu
zebrafishWB

Cancer Sci

Dhx15 regulates zebrafish definitive hematopoiesis through the unfolded protein response pathway.

Authors - Yuanhua Cai
humanWB

J Cell Mol Med

ETS1 and SP1 drive DHX15 expression in acute lymphoblastic leukaemia.

Authors - Xiang-Lei Chen
humanWB

FEBS Lett

Tumor suppressor RBM5 directly interacts with the DExD/H-box protein DHX15 and stimulates its helicase activity.

Authors - Niu Zhaoyang Z
humanWB

RNA

A model for DHX15 mediated disassembly of A-complex spliceosomes

Authors - Hannah M Maul-Newby

PLoS Pathog

Downregulation of CPSF6 Leads to Global mRNA 3' UTR shortening and enhanced antiviral immune responses

Authors - Yong Ge
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