• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

DHX15 Recombinant antibody, PBS Only

DHX15 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F13

Cat no : 82137-1-PBS

Print datasheet

Synonyms

EC:3.6.4.13, DDX15, DBP1, ATP-dependent RNA helicase DHX15, ATP-dependent RNA helicase #46

验证数据展示

  • WB analysis using 82137-1-RR (same clone as 82137-1-PBS)

    WB analysis using 82137-1-RR (same clone as 82137-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82137-1-RR (DHX15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82137-1-RR (same clone as 82137-1-PBS)

    WB analysis of HeLa using 82137-1-RR (same clone as 82137-1-PBS)

    WB result of DHX15 antibody (82137-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX15 transfected HeLa cells. This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

  • IP experiment of NIH/3T3 using 82137-1-RR (same clone as 82137-1-PBS)

    IP experiment of NIH/3T3 using 82137-1-RR (same clone as 82137-1-PBS)

    IP result of anti-DHX15 (IP:82137-1-RR, 4ug; Detection:82137-1-RR 1:8000) with NIH/3T3 cells lysate 1320 ug. This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 82137-1-RR (same clone as 82137-1-PBS)

    IHC staining of mouse testis using 82137-1-RR (same clone as 82137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82137-1-RR (DHX15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 82137-1-RR (same clone as 82137-1-PBS)

    IHC staining of mouse testis using 82137-1-RR (same clone as 82137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82137-1-RR (DHX15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82137-1-RR (same clone as 82137-1-PBS)

    IF Staining of HeLa using 82137-1-RR (same clone as 82137-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DHX15 antibody (82137-1-RR, Clone: 1F13 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82137-1-RR (same clone as 82137-1-PBS)

    FC experiment of HeLa using 82137-1-RR (same clone as 82137-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DHX15 (82137-1-RR, Clone:1F13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82137-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82137-1-RR

    Affinity and Kinetic Characterization of 82137-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82137-1-RR against Human DHX15 were performed. The affinity constant is below 1 pM.

产品信息

82137-1-PBS targets DHX15 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DHX15 fusion protein Ag2895 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 15
Synonyms EC:3.6.4.13, DDX15, DBP1, ATP-dependent RNA helicase DHX15, ATP-dependent RNA helicase #46
Calculated Molecular Weight 795 aa, 91 kDa
Observed Molecular Weight90-95 kDa
GenBank Accession NumberBC035974
Gene Symbol DHX15
Gene ID (NCBI) 1665
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43143
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DHX15, also named as DBP1 and DDX15, is a DExD/H-box protein that has been identified in spliceosome, through its carboxyl (C)-terminal G-patch domain and stimulates the helicase activity of DHX15. DHX15 is a pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. The MW of DHX15 is about 90-95kDa.