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DHX29 Polyclonal antibody

DHX29 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13923-1-AP

Print datasheet

Synonyms

Nucleic acid helicase DDXx, EC:3.6.4.13, DEAH box protein 29, DDX29, ATP-dependent RNA helicase DHX29

验证数据展示

  • WB analysis of HEK-293 using 13923-1-AP

    WB analysis of HEK-293 using 13923-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13923-1-AP (DHX29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 13923-1-AP

    IHC staining of human ovary cancer using 13923-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13923-1-AP (DHX29 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HT-1080 cells, K-562 cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13923-1-AP targets DHX29 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DHX29 fusion protein Ag4938 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 29
Synonyms Nucleic acid helicase DDXx, EC:3.6.4.13, DEAH box protein 29, DDX29, ATP-dependent RNA helicase DHX29
Calculated Molecular Weight 155 kDa
Observed Molecular Weight 150-155 kDa
GenBank Accession NumberBC056219
Gene Symbol DHX29
Gene ID (NCBI) 54505
RRIDAB_2092005
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z478
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DHX29 antibody 13923-1-APDownload protocol
IHC protocol for DHX29 antibody 13923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols