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DHX32 Polyclonal antibody
DHX32 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, mouse colon tissue, A431 cells, COLO 320 cells |
| Positive IHC detected in | human testis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 2 publications below |
产品信息
19808-1-AP targets DHX32 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | DHX32 fusion protein Ag13834 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | DEAH (Asp-Glu-Ala-His) box polypeptide 32 |
| Synonyms | DDX32, DEAD/H box 32, DEAD/H helicase like protein 1, DEAH box protein 32, DHLP1, DHX32, HuDDX32 |
| Calculated Molecular Weight | 743 aa, 84 kDa |
| Observed Molecular Weight | 84 kDa, 75 kDa |
| GenBank Accession Number | BC002473 |
| Gene Symbol | DHX32 |
| Gene ID (NCBI) | 55760 |
| RRID | AB_10641031 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7L7V1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DHX32, a new member of the DEAH subfamily of DEAD box proteins, is a novel putative RNA helicase with an activation-dependent paten of expression and characterized by a conserved helicase domain consisting of 7 to 8 conserved motifs. RNA helicases of the DEAD box family are highly conserved enzymes that are required for RNA metabolism from transcription to degradation and are therefore important players in gene expression. DHX32 may play an important role in the development of colorectal cancer. This is a rabbit polyclonal anti-DHX32 antibody raised against part of the C-terminal chain of human DHX32.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DHX32 antibody 19808-1-AP | Download protocol |
| IHC protocol for DHX32 antibody 19808-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Transl Cancer Res Silencing of DHX32 increases the proliferation of liver cancer cells.
|
