验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, mouse colon tissue, A431 cells, COLO 320 cells |
| Positive IHC detected in | human testis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 2 publications below |
产品信息
19808-1-AP targets DHX32 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | DHX32 fusion protein Ag13834 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DEAH (Asp-Glu-Ala-His) box polypeptide 32 |
| 别名 | DDX32, DEAD/H box 32, DEAD/H helicase like protein 1, DEAH box protein 32, DHLP1, DHX32, HuDDX32 |
| 计算分子量 | 743 aa, 84 kDa |
| 观测分子量 | 84 kDa, 75 kDa |
| GenBank蛋白编号 | BC002473 |
| 基因名称 | DHX32 |
| Gene ID (NCBI) | 55760 |
| RRID | AB_10641031 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7L7V1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DHX32, a new member of the DEAH subfamily of DEAD box proteins, is a novel putative RNA helicase with an activation-dependent paten of expression and characterized by a conserved helicase domain consisting of 7 to 8 conserved motifs. RNA helicases of the DEAD box family are highly conserved enzymes that are required for RNA metabolism from transcription to degradation and are therefore important players in gene expression. DHX32 may play an important role in the development of colorectal cancer. This is a rabbit polyclonal anti-DHX32 antibody raised against part of the C-terminal chain of human DHX32.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DHX32 antibody 19808-1-AP | Download protocol |
| IHC protocol for DHX32 antibody 19808-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
