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验证数据展示

  • WB analysis of HeLa using 24525-1-AP

    WB analysis of HeLa using 24525-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24525-1-AP (DHX57 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 24525-1-AP

    IHC staining of mouse testis using 24525-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24525-1-AP (DHX57 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 24525-1-AP

    IHC staining of mouse testis using 24525-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24525-1-AP (DHX57 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 24525-1-AP

    IF Staining of HeLa using 24525-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DHX57 antibody (24525-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HeLa using 24525-1-AP

    IF Staining of HeLa using 24525-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 24525-1-AP (DHX57 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

DHX57 Polyclonal antibody

DHX57 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24525-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Putative ATP-dependent RNA helicase DHX57, EC:3.6.4.13, DDX57

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24525-1-AP targets DHX57 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 DHX57 fusion protein Ag21743 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DEAH (Asp-Glu-Ala-Asp/His) box polypeptide 57
别名 Putative ATP-dependent RNA helicase DHX57, EC:3.6.4.13, DDX57
计算分子量 1386 aa, 156 kDa
观测分子量 150 kDa
GenBank蛋白编号BC131534
基因名称 DHX57
Gene ID (NCBI) 90957
RRIDAB_2879590
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6P158
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DEAH (Asp-Glu-Ala-Asp/His) box polypeptide 57 (DHX57), also named as DDX57, is a member of the DEAH subfamily within the Dead box protein family which is involved in modifications of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. This antibody reacts with 150 kDa DHX57 protein.

实验方案

Product Specific Protocols
WB protocol for DHX57 antibody 24525-1-APDownload protocol
IHC protocol for DHX57 antibody 24525-1-APDownload protocol
IF protocol for DHX57 antibody 24525-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols