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  • Featured Product
  • KD/KO Validated

DHX9 Monoclonal antibody, PBS Only (Detector)

DHX9 Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA, Cytometric bead array

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IP, IF, IHC, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

1B12C10

Cat No : 67153-1-PBS

Print datasheet

Synonyms

ATP dependent RNA helicase A, DDX9, DEAH box protein 9, DHX9, LKP, NDH II, NDH2, NDHII, Nuclear DNA helicase II, RHA

验证数据展示

  • WB analysis using 67153-1-Ig (same clone as 67153-1-PBS)

    WB analysis using 67153-1-Ig (same clone as 67153-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67153-1-Ig (DHX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 67153-1-Ig (same clone as 67153-1-PBS)

    IP experiment of HeLa using 67153-1-Ig (same clone as 67153-1-PBS)

    IP result of anti-DHX9 (IP:67153-1-Ig, 5ug; Detection:67153-1-Ig 1:20000) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67153-1-Ig (same clone as 67153-1-PBS)

    IHC staining of mouse brain using 67153-1-Ig (same clone as 67153-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67153-1-Ig (DHX9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DHX9 antibody included
  • IF Staining of HepG2 using 67153-1-Ig (same clone as 67153-1-PBS)

    IF Staining of HepG2 using 67153-1-Ig (same clone as 67153-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67153-1-Ig (DHX9 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67153-1-Ig (same clone as 67153-1-PBS)

    WB analysis of HEK-293 using 67153-1-Ig (same clone as 67153-1-PBS)

    WB result of DHX9 antibody (67153-1-Ig; 1:400000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX9 transfected HEK-293 cells. This data was developed using the same antibody clone with 67153-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50161-1

    Cytometric bead array standard curve of MP50161-1

    Cytometric bead array standard curve of MP50161-1, DHX9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67153-2-PBS. Detection antibody: 67153-1-PBS. Standard:Ag12104. Range: 1.563-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy DHX9 Ready-To-Use IHC Kit. DHX9 primary antibody included.

产品信息

67153-1-PBS targets DHX9 as part of a matched antibody pair:

MP50161-1: 67153-2-PBS capture and 67153-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IP, IF, IHC, Indirect ELISA, Cytometric bead array Application Description
Tested Reactivity Human, mouse, rat
Immunogen DHX9 fusion protein Ag12104 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 9
Synonyms ATP dependent RNA helicase A, DDX9, DEAH box protein 9, DHX9, LKP, NDH II, NDH2, NDHII, Nuclear DNA helicase II, RHA
Calculated Molecular Weight 1270 aa, 141 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC014246
Gene Symbol DHX9
Gene ID (NCBI) 1660
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08211
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

RNA helicases play important roles in transcription, RNA processing, translation, and RNA replication. DEAD box proteins are putative RNA helicases that have a characteristic Asp-Glu-Ala-Asp (DEAD) box as 1 of 8 highly conserved sequence motifs. DHX9 a member of the DEAH family of proteins, which possess a double-stranded RNA-binding domain (dsRBD) and a helicase domain [PMID:20569003]. It unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure of DHX9 may subsequently influence interactions with proteins or other nucleic acids. It is also a component of the CRD-mediated complex that promotes MYC mRNA stability. In addition, it is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2[PMID: 19029303, 22190748].