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  • Featured Product
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Smac/DIABLO Polyclonal antibody

Smac/DIABLO Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10434-1-AP

Print datasheet

Synonyms

DIABLO, DFNA64, Direct IAP-binding protein with low pI, Diablo IAP-binding mitochondrial protein, cleaved form, Diablo IAP-binding mitochondrial protein

验证数据展示

  • WB analysis using 10434-1-AP

    WB analysis using 10434-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10434-1-AP (DIABLO antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 10434-1-AP

    WB analysis of HeLa using 10434-1-AP

    WB result of DIABLO antibody (10434-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIABLO transfected HeLa cells.

  • IP experiment of HepG2 using 10434-1-AP

    IP experiment of HepG2 using 10434-1-AP

    IP result of anti-DIABLO (IP:10434-1-AP, 4ug; Detection:10434-1-AP 1:2000) with HepG2 cells lysate 880 ug.

  • IHC staining of mouse testis using 10434-1-AP

    IHC staining of mouse testis using 10434-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10434-1-AP (DIABLO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 10434-1-AP

    IF Staining of HeLa using 10434-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DIABLO antibody (10434-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HeLa using 10434-1-AP

    FC experiment of HeLa using 10434-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DIABLO (10434-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, mouse testis tissue, rat testis tissue
Positive IP detected inHepG2 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10434-1-AP targets Smac/DIABLO in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, hamster
Immunogen Smac/DIABLO fusion protein Ag0696 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name diablo homolog (Drosophila)
Synonyms DIABLO, DFNA64, Direct IAP-binding protein with low pI, Diablo IAP-binding mitochondrial protein, cleaved form, Diablo IAP-binding mitochondrial protein
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC011909
Gene Symbol DIABLO
Gene ID (NCBI) 56616
RRIDAB_2092647
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NR28
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Smac/DIABLO is a mitochondrial protein that promotes apoptosis by neutralizing members of the IAP family. SMAC/DIABLO is ubiquitously expressed in normal tissues, and the expression of SMAC messenger RNA (mRNA) in adult testis is the highest .Smac/DIABLO encodes a 239 amino acid protein (~27 kDa). The first 55 amino acids are a mitochondrial targeting signal peptide that is cleaved to generate mature Smac-DIABLO (~21 kDa). When released from mitochondria, Smac-DIABLO acts as a natural antagonist of inhibitor of apoptosis proteins (IAPs). Smac-DIABLO antagonistic activity is based on its N-terminal tetrapeptide (AVPI) that binds baculoviral IAP repeat (BIR) domains of IAPs, releasing their inhibitory effects on both initiator and effector caspases, thus promoting cell death. (PMID: 32325691, PMID: 32575872,PMID: 25650938)

实验方案

Product Specific Protocols
WB protocol for Smac/DIABLO antibody 10434-1-APDownload protocol
IHC protocol for Smac/DIABLO antibody 10434-1-APDownload protocol
IF protocol for Smac/DIABLO antibody 10434-1-APDownload protocol
IP protocol for Smac/DIABLO antibody 10434-1-APDownload protocol
FC protocol for Smac/DIABLO antibody 10434-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Eur J Med Chem

PKM2/PDK1 dual-targeted shikonin derivatives restore the sensitivity of EGFR-mutated NSCLC cells to gefitinib by remodeling glucose metabolism

Authors - Hongyan Lin
View Article
mouseWB

J Cell Physiol

Resveratrol ameliorates podocyte damage in diabetic mice via SIRT1/PGC-1α mediated attenuation of mitochondrial oxidative stress.

Authors - Tao Zhang
View Article
WB

Ann Transl Med

Botrychium schaffneri Underw. extract acts via DIABLO to induce apoptosis and inhibit proliferation of non-small cell lung carcinoma in vitro and in vivo.

Authors - Yu-Jia Liu
View Article
humanWB

Chin Med

Shikonin and 4-hydroxytamoxifen synergistically inhibit the proliferation of breast cancer cells through activating apoptosis signaling pathway in vitro and in vivo.

Authors - Hong-Yan Lin
View Article
humanWB

Acta Biochim Biophys Sin (Shanghai)

Cathelicidin PR-39 peptide inhibits hypoxia/reperfusion-induced kidney cell apoptosis by suppression of the endoplasmic reticulum-stress pathway.

Authors - Jing Liu
View Article
ratIHC

Mol Med Rep

Mechanisms underlying the protective effect of tannic acid against arsenic trioxide‑induced cardiotoxicity in rats: Potential involvement of mitochondrial apoptosis.

Authors - Yucong Xue
View Article