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验证数据展示

  • WB analysis of HeLa using 20567-1-AP (same clone as 20567-1-PBS)

    WB analysis of HeLa using 20567-1-AP (same clone as 20567-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20567-1-AP (DICER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 20567-1-AP (same clone as 20567-1-PBS)

    WB analysis of HepG2 using 20567-1-AP (same clone as 20567-1-PBS)

    WB result of DICER1 antibody (20567-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-DICER1 transfected HepG2 cells. This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 20567-1-AP (same clone as 20567-1-PBS)

    IP experiment of Jurkat using 20567-1-AP (same clone as 20567-1-PBS)

    IP result of anti-DICER1 (IP:20567-1-AP, 5ug; Detection:20567-1-AP 1:300) with Jurkat cells lysate 2000ug. This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 20567-1-AP (same clone as 20567-1-PBS)

    IHC staining of human testis using 20567-1-AP (same clone as 20567-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20567-1-AP (DICER1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

  • IF Staining of human ovary tumor using 20567-1-AP (same clone as 20567-1-PBS)

    IF Staining of human ovary tumor using 20567-1-AP (same clone as 20567-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using DICER1 antibody (20567-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

  • IF Staining of human ovary tumor using 20567-1-AP (same clone as 20567-1-PBS)

    IF Staining of human ovary tumor using 20567-1-AP (same clone as 20567-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using DICER1 antibody (20567-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 20567-1-AP (same clone as 20567-1-PBS)

    IF Staining of HepG2 using 20567-1-AP (same clone as 20567-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DICER1 antibody (20567-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 20567-1-AP (same clone as 20567-1-PBS)

    FC experiment of HepG2 using 20567-1-AP (same clone as 20567-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human DICER1 (20567-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20567-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

DICER1 Polyclonal antibody, PBS Only

DICER1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Cat No. 20567-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

20567-1-PBS targets DICER1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dicer 1, ribonuclease type III
别名
计算分子量 219 kDa
观测分子量 220-250 kDa, 90 kDa
GenBank蛋白编号NM_030621
基因名称 DICER1
Gene ID (NCBI) 23405
RRIDAB_10694287
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPY3
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DICER1, also named as DICER, HERNA and KIAA0928, belongs to the helicase family and Dicer subfamily. It is required for formation of the RNA induced silencing complex (RISC). DICER1 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. DICER1 cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs. The antibody is specific to DICER1. The calcualted molecular weight of DICER1 is 219 kDa, but modified DICER1 is about 220-250 kDa. DICER1 exists some isoforms with molecular weight 219 kDa and 93 kDa.