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DICER1 Polyclonal antibody

DICER1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29984-1-AP

Print datasheet

Synonyms

HERNA, Helicase MOI, Endoribonuclease Dicer, EC:3.1.26.3, DICER

验证数据展示

  • WB analysis using 29984-1-AP

    WB analysis using 29984-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29984-1-AP (DICER1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 29984-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 29984-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29984-1-AP (DICER1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29984-1-AP targets DICER1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen DICER1 fusion protein Ag30368 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dicer 1, ribonuclease type III
Synonyms HERNA, Helicase MOI, Endoribonuclease Dicer, EC:3.1.26.3, DICER
Calculated Molecular Weight 219 kDa
Observed Molecular Weight90 kDa, 220-250 kDa
GenBank Accession NumberNM_030621
Gene Symbol DICER1
Gene ID (NCBI) 23405
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPY3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DICER1 functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. DICER1 cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, and producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation.

实验方案

Product Specific Protocols
WB protocol for DICER1 antibody 29984-1-APDownload protocol
IHC protocol for DICER1 antibody 29984-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols