验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, MCF-7 cells |
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy DIO2 Ready-To-Use IHC Kit. DIO2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 12 publications below |
| IHC | See 2 publications below |
产品信息
26513-1-AP targets DIO2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | DIO2 fusion protein Ag24119 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | deiodinase, iodothyronine, type II |
| 别名 | 5DII, D2, DIO2, DIOII, ITDI2, SelY, TXDI2, Type 2 DI, Type II 5 deiodinase |
| 计算分子量 | 273 aa, 31 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC074882 |
| 基因名称 | DIO2 |
| Gene ID (NCBI) | 1734 |
| RRID | AB_2880538 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92813 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Type II iodothyronine deiodinase(DIO2), belongs to the iodothyronine deiodinase family, with two isoform of 30 and 34 kDa. DIO2 can activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). DIO2 is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DIO2 antibody 26513-1-AP | Download protocol |
| IHC protocol for DIO2 antibody 26513-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Tumor Cell "Slimming" Regulates Tumor Progression through PLCL1/UCP1-Mediated Lipid Browning. | ||
Nat Chem Biol Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability. | ||
Diabetes Id1 Promotes Obesity by Suppressing Brown Adipose Thermogenesis and White Adipose Browning. | ||
Clin Transl Med NNT-induced tumor cell "slimming" reverses the pro-carcinogenesis effect of HIF2a in tumors. | ||
FASEB J The tumor secretory factor ZAG promotes white adipose tissue browning and energy wasting. | ||
Ecotoxicol Environ Saf Exposure to melamine cyanuric acid in adult mice induced thyroid dysfunction and circadian rhythm disorder. |
