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  • Featured Product
  • KD/KO Validated

DIRAS2 Polyclonal antibody

DIRAS2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15557-1-AP

Print datasheet

Synonyms

GTP-binding protein Di-Ras2, GTP binding protein Di Ras2

验证数据展示

  • WB analysis of human brain using 15557-1-AP

    WB analysis of human brain using 15557-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 15557-1-AP

    IP experiment of mouse brain using 15557-1-AP

    IP result of anti-DIRAS2 (IP:15557-1-AP, 4ug; Detection:15557-1-AP 1:1000) with mouse brain tissue lysate 4400ug.

  • IF Staining of HeLa using 15557-1-AP

    IF Staining of HeLa using 15557-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15557-1-AP (DIRAS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman brain tissue, rat brain tissue, HeLa cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15557-1-AP targets DIRAS2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen DIRAS2 fusion protein Ag7926 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DIRAS family, GTP-binding RAS-like 2
Synonyms GTP-binding protein Di-Ras2, GTP binding protein Di Ras2
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC008065
Gene Symbol DIRAS2
Gene ID (NCBI) 54769
RRIDAB_2261563
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96HU8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DIRAS2 antibody 15557-1-APDownload protocol
IF protocol for DIRAS2 antibody 15557-1-APDownload protocol
IP protocol for DIRAS2 antibody 15557-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Oncogene

Di-Ras2 promotes renal cell carcinoma formation by activating the mitogen-activated protein kinase pathway in the absence of von Hippel-Lindau protein.

Authors - Hanyu Rao
  • KD Validated
View Article
humanWB

Cancers (Basel)

Frequent Epigenetic Inactivation of DIRAS-1 and DIRAS-2 Contributes to Chemo-Resistance in Gliomas.

Authors - Tanja Rothhammer-Hampl
View Article
human,mouseWB,IF

J Neural Transm (Vienna)

Expression of the ADHD candidate gene Diras2 in the brain.

Authors - Lena Grünewald
View Article
humanWB

Dev Cell

The UBE2F-CRL5ASB11-DIRAS2 axis is an oncogene and tumor suppressor cascade in pancreatic cancer cells

Authors - Yu Chang
View Article