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DIS3 Polyclonal antibody

DIS3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14689-1-AP

Print datasheet

Synonyms

KIAA1008, EXOSC11, EC:3.1.13.-, dis3p, Dis-3



经过测试的应用

Positive WB detected inHEK-293T cells, mouse ovary tissue, mouse testis tissue, HeLa cells, Jurkat cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DIS3 Ready-To-Use IHC Kit. DIS3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14689-1-AP targets DIS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, drosophila
Immunogen DIS3 fusion protein Ag6117 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DIS3 mitotic control homolog (S. cerevisiae)
Synonyms KIAA1008, EXOSC11, EC:3.1.13.-, dis3p, Dis-3
Calculated Molecular Weight 109 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC056143
Gene Symbol DIS3
Gene ID (NCBI) 22894
RRIDAB_2091025
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2L1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DIS3, also named as KIAA1008 and RRP44, belongs to the ribonuclease II (RNB) family. It is a component of the exosome 3'->5' exoribonuclease complex. DIS3 is required for the 3'-processing of the 7S pre-RNA to the mature nuclear complex. It is also associated with the GTPase Ran. DIS3 has a 3'-5' exonuclease activity.

实验方案

Product Specific Protocols
WB protocol for DIS3 antibody 14689-1-APDownload protocol
IHC protocol for DIS3 antibody 14689-1-APDownload protocol
IF protocol for DIS3 antibody 14689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

A role for the Perlman syndrome exonuclease Dis3l2 in the Lin28-let-7 pathway.

Authors - Chang Hao-Ming HM
humanWB

Mol Cell

DGCR8 Acts as an Adaptor for the Exosome Complex to Degrade Double-Stranded Structured RNAs.

Authors - Sara Macias
mouseWB

Nucleic Acids Res

RNA-regulatory exosome complex confers cellular survival to promote erythropoiesis.

Authors - Charu Mehta
mouseIF

Proc Natl Acad Sci U S A

The early macrophage response to pathogens requires dynamic regulation of the nuclear paraspeckle

Authors - Sikandar Azam
  • KD Validated
humanWB

EMBO J

The RNA exosome complex degrades expanded hexanucleotide repeat RNA in C9orf72 FTLD/ALS.

Authors - Yuya Kawabe
  • KD Validated
mouseWB

Int J Biol Sci

Photobiomodulation Drives MiR-136-5p Expression to Promote Injury Repair after Myocardial Infarction.

Authors - Xinlu Gao