验证数据展示
经过测试的应用
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
15500-1-AP targets DISC1-long-specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | disrupted in schizophrenia 1 |
| 别名 | C1orf136, DISC1, DISC1 long, DISC1-long-specific, disrupted in schizophrenia 1, KIAA0457, SCZD9 |
| 计算分子量 | 95 kDa |
| GenBank蛋白编号 | NM_018662 |
| 基因名称 | DISC1 |
| Gene ID (NCBI) | 27185 |
| RRID | AB_2230451 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NRI5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DISC1, also named as KIAA0457, is identified as the sole gene whose ORF is truncated and cosegregates with major mental illnesses in a Scottish family. DISC1 may be implicated in psychiatric conditions in other populations than the unique Scottish family. It is involved in the regulation of multiple aspects of embryonic and adult neurogenesis. DISC1 is required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus. It plays a role, together with PCNT, in the microtubule network formation. In WB, DISC1 has some MW such as 34kd(PMID:18620078 );75-85kd and 95-100kd(PMID:15657124). DISC1 isoforms have distinct developmental patterns of expression. This antibody is against C-terminal of DISC1, binding full length protein specifically.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for DISC1-long-specific antibody 15500-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Neurosci Lack of Helios During Neural Development Induces Adult Schizophrenia-Like Behaviors Associated With Aberrant Levels of the TRIF-Recruiter Protein WDFY1. | ||
Neurobiol Dis Hippocampal Pyk2 regulates specific social skills: Implications for schizophrenia |