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DISP1 Polyclonal antibody

DISP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12041-1-AP

Print datasheet

Synonyms

DISP1, DISPA, Protein dispatched homolog 1

验证数据展示

  • WB analysis using 12041-1-AP

    WB analysis using 12041-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12041-1-AP (DISP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 12041-1-AP

    IHC staining of human kidney using 12041-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12041-1-AP (DISP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 12041-1-AP

    IHC staining of human kidney using 12041-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12041-1-AP (DISP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12041-1-AP targets DISP1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen DISP1 fusion protein Ag2670 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dispatched homolog 1 (Drosophila)
Synonyms DISP1, DISPA, Protein dispatched homolog 1
Calculated Molecular Weight 1524 aa, 171 kDa
Observed Molecular Weight 150-171 kDa
GenBank Accession NumberBC011542
Gene Symbol DISP1
Gene ID (NCBI) 84976
RRIDAB_2091185
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96F81
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DISP1 antibody 12041-1-APDownload protocol
IHC protocol for DISP1 antibody 12041-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols