验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, COLO 320 cells, Jurkat cells, MCF-7 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy DKC1 Ready-To-Use IHC Kit. DKC1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25420-1-AP targets DKC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | DKC1 fusion protein Ag7992 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dyskeratosis congenita 1, dyskerin |
| 别名 | CBF5, CBF5 homolog, DKC, DKC1, Dyskerin, NAP57, NOLA4, Nucleolar protein NAP57, snoRNP protein DKC1, XAP101 |
| 计算分子量 | 58 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC009928 |
| 基因名称 | DKC1 |
| Gene ID (NCBI) | 1736 |
| RRID | AB_2880071 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60832 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DKC1 antibody 25420-1-AP | Download protocol |
| IHC protocol for DKC1 antibody 25420-1-AP | Download protocol |
| IF protocol for DKC1 antibody 25420-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Stem Cells H/ACA Box Small Nucleolar RNA 7A promotes the Self-Renewal of Human Umbilical Cord Mesenchymal Stem Cells. | ||
Cell Rep Chromatin-associated orphan snoRNA regulates DNA damage-mediated differentiation via a non-canonical complex.
| ||
J Cancer Res Clin Oncol Identifying and validating the roles of the cuproptosis-related gene DKC1 in cancer with a focus on esophageal carcinoma
|
