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DKK3 Monoclonal antibody, PBS Only

DKK3 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

4E6H6

Cat No : 66758-1-PBS

Print datasheet

Synonyms

Dickkopf 3, Dickkopf related protein 3, Dkk 3, DKK3, hDkk 3, REIC, RIG

验证数据展示

  • WB analysis using 66758-1-Ig (same clone as 66758-1-PBS)

    WB analysis using 66758-1-Ig (same clone as 66758-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66758-1-Ig (DKK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66758-1-Ig (same clone as 66758-1-PBS)

    IHC staining of human heart using 66758-1-Ig (same clone as 66758-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66758-1-Ig (DKK3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DKK3 antibody included
  • IF Staining of HEK-293 using 66758-1-Ig (same clone as 66758-1-PBS)

    IF Staining of HEK-293 using 66758-1-Ig (same clone as 66758-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DKK3 antibody (66758-1-Ig, Clone: 4E6H6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy DKK3 Ready-To-Use IHC Kit. DKK3 primary antibody included.

产品信息

66758-1-PBS targets DKK3 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen DKK3 fusion protein Ag23615 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name dickkopf homolog 3 (Xenopus laevis)
Synonyms Dickkopf 3, Dickkopf related protein 3, Dkk 3, DKK3, hDkk 3, REIC, RIG
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 40 kDa, 50-55 kDa
GenBank Accession NumberBC007660
Gene Symbol DKK3
Gene ID (NCBI) 27122
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UBP4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

DKK3, also named as REIC, belongs to the dickkopf family. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer's disea. The adenovirus carrying REIC/Dkk-3 (Ad-REIC) exhibits a potent tumor-specific cell-killing function for various human cancers. It has also become evident that some human cancers are resistant to Ad-REIC-induced apoptosis. The DKK3 is a Glycosylation protein with MW about 55 kDa or 38-43 kDa.