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DKK4 Polyclonal antibody

DKK4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 27080-1-AP

Print datasheet

Synonyms

Dickkopf 4, Dickkopf related protein 4, DKK 4, DKK4, hDkk 4

验证数据展示

  • WB analysis of mouse cerebellum using 27080-1-AP

    WB analysis of mouse cerebellum using 27080-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 27080-1-AP (DKK4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human oesophagus using 27080-1-AP

    IHC staining of human oesophagus using 27080-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 27080-1-AP (DKK4 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human oesophagus using 27080-1-AP

    IHC staining of human oesophagus using 27080-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 27080-1-AP (DKK4 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse cerebellum tissue
Positive IHC detected inhuman oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 27080-1-AP is DKK4 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen DKK4 fusion protein Ag24209 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dickkopf homolog 4 (Xenopus laevis)
Synonyms Dickkopf 4, Dickkopf related protein 4, DKK 4, DKK4, hDkk 4
Calculated Molecular Weight 224 aa, 25 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC107047
Gene Symbol DKK4
Gene ID (NCBI) 27121
RRIDAB_2880746
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBT3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DKK4 antibody 27080-1-APDownload protocol
IHC protocol for DKK4 antibody 27080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Clin Transl Med

O-GlcNAcylated LARP1 positively regulated by circCLNS1A facilitates hepatoblastoma progression through DKK4/β-catenin signalling

Authors - Zhongqi Cui
View Article