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DLAT Polyclonal antibody

DLAT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 13426-1-AP

Print datasheet

Synonyms

PDC-E2, ODP2, EC:2.3.1.12, DLTA, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

验证数据展示

  • WB analysis using 13426-1-AP

    WB analysis using 13426-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13426-1-AP (DLAT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 13426-1-AP

    WB analysis of HeLa using 13426-1-AP

    WB result of DLAT antibody (13426-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLAT transfected HeLa cells.

  • WB analysis using 13426-1-AP

    WB analysis using 13426-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13426-1-AP (DLAT antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 13426-1-AP

    IHC staining of human liver cancer using 13426-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DLAT antibody included
  • IHC staining of human liver cancer using 13426-1-AP

    IHC staining of human liver cancer using 13426-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DLAT antibody included
  • IF Staining of HepG2 using 13426-1-AP

    IF Staining of HepG2 using 13426-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (13426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of mouse kidney using 13426-1-AP

    IHC staining of mouse kidney using 13426-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DLAT antibody included
  • IHC staining of mouse kidney using 13426-1-AP

    IHC staining of mouse kidney using 13426-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DLAT antibody included
  • IP experiment of HepG2 using 13426-1-AP

    IP experiment of HepG2 using 13426-1-AP

    IP result of anti-DLAT (IP:13426-1-AP, 3ug; Detection:13426-1-AP 1:1000) with HepG2 cells lysate 2000ug.

  • IHC staining of human colon cancer using 13426-1-AP

    IHC staining of human colon cancer using 13426-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DLAT antibody included
  • IF Staining of HepG2 using 13426-1-AP

    IF Staining of HepG2 using 13426-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DLAT antibody (13426-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, A431 cells, HepG2 cells, Jurkat cells, LNCaP cells, MCF-7 cells, HEK-293 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DLAT Ready-To-Use IHC Kit. DLAT primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13426-1-AP targets DLAT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit
Immunogen DLAT fusion protein Ag4226 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dihydrolipoamide S-acetyltransferase
Synonyms PDC-E2, ODP2, EC:2.3.1.12, DLTA, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Calculated Molecular Weight 647 aa, 70 kDa
Observed Molecular Weight 69-70 kDa
GenBank Accession NumberBC039084
Gene Symbol DLAT
Gene ID (NCBI) 1737
RRIDAB_2091774
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10515
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DLAT antibody 13426-1-APDownload protocol
IHC protocol for DLAT antibody 13426-1-APDownload protocol
IF protocol for DLAT antibody 13426-1-APDownload protocol
IP protocol for DLAT antibody 13426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Res

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

Authors - Yunzi Mao
View Article
mouseWB

Nat Cell Biol

RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis

Authors -
View Article
human,mouseWB,IF,CoIP

Mol Cell

Acetyl-CoA production by Mediator-bound 2-ketoacid dehydrogenases boosts de novo histone acetylation and is regulated by nitric oxide

Authors - Marta Russo
View Article
mouseWB,IF

Adv Healthc Mater

Biomimetic Nano-regulator that Induces Cuproptosis and Lactate-Depletion Mediated ROS Storm for Metalloimmunotherapy of Clear Cell Renal Cell Carcinoma

Authors - Xiaotong Xu
View Article
mouseWB,IF

Adv Healthc Mater

Copper-Based Composites Nanoparticles Improve Triple-Negative Breast Cancer Treatment with Induction of Apoptosis-Cuproptosis and Immune Activation

Authors - Ning Wang
View Article
ratWB,IF

Sci Total Environ

TRIM24-DTNBP1-ATP7A mediated astrocyte cuproptosis in cognition and memory dysfunction caused by Y2O3 NPs

Authors - Ziwei Chen
View Article