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DLAT Monoclonal antibody

DLAT Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1F2B9

Cat no : 68303-1-Ig

Print datasheet

Synonyms

PDC-E2, ODP2, EC:2.3.1.12, DLTA, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

验证数据展示

  • IF Staining of HepG2 using 68303-1-Ig

    IF Staining of HepG2 using 68303-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (68303-1-Ig, Clone: 1F2B9 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • WB analysis using 68303-1-Ig

    WB analysis using 68303-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68303-1-Ig (DLAT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 68303-1-Ig

    IHC staining of human liver cancer using 68303-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68303-1-Ig (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DLAT antibody included
  • IHC staining of human liver cancer using 68303-1-Ig

    IHC staining of human liver cancer using 68303-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68303-1-Ig (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DLAT antibody included
  • IHC staining of mouse kidney using 68303-1-Ig

    IHC staining of mouse kidney using 68303-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68303-1-Ig (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DLAT antibody included
  • IHC staining of mouse kidney using 68303-1-Ig

    IHC staining of mouse kidney using 68303-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68303-1-Ig (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DLAT antibody included
  • IF Staining of HepG2 using 68303-1-Ig

    IF Staining of HepG2 using 68303-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (68303-1-Ig, Clone: 1F2B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHCT 116 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman liver cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DLAT Ready-To-Use IHC Kit. DLAT primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68303-1-Ig targets DLAT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DLAT fusion protein Ag4226 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name dihydrolipoamide S-acetyltransferase
Synonyms PDC-E2, ODP2, EC:2.3.1.12, DLTA, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Calculated Molecular Weight 647 aa, 70 kDa
Observed Molecular Weight 69-70 kDa
GenBank Accession NumberBC039084
Gene Symbol DLAT
Gene ID (NCBI) 1737
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10515
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DLAT antibody 68303-1-IgDownload protocol
IHC protocol for DLAT antibody 68303-1-IgDownload protocol
IF protocol for DLAT antibody 68303-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Immunol

The signature of cuproptosis-related immune genes predicts the tumor microenvironment and prognosis of prostate adenocarcinoma

Authors - Kai Yao
View Article
humanIF

Acta Biomater

Copper-Coordinated Nanoassemblies based on Photosensitizer-Chemo Prodrugs and Checkpoint Inhibitors for Enhanced Apoptosis-Cuproptosis and Immunotherapy

Authors - Wenlong Liang
View Article