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DLC1 Polyclonal antibody

DLC1 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15460-1-AP

Print datasheet

Synonyms

HP protein, HP, DLC-1, DLC 1, deleted in liver cancer 1

验证数据展示

  • IHC staining of human spleen using 15460-1-AP

    IHC staining of human spleen using 15460-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15460-1-AP (DLC1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human spleen using 15460-1-AP

    IHC staining of human spleen using 15460-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15460-1-AP (DLC1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 15460-1-AP

    IF Staining of HepG2 using 15460-1-AP

    Immunofluorescent analysis of HepG2 cells, using DLC1 antibody 15460-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of mouse lung using 15460-1-AP

    IP experiment of mouse lung using 15460-1-AP

    IP result of anti-DLC1 (IP:15460-1-AP, 4ug; Detection:15460-1-AP 1:300) with mouse lung tissue lysate 2400ug.

经过测试的应用

Positive IP detected inmouse lung tissue
Positive IHC detected inhuman spleen tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15460-1-AP targets DLC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DLC1 fusion protein Ag7732 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deleted in liver cancer 1
Synonyms HP protein, HP, DLC-1, DLC 1, deleted in liver cancer 1
Calculated Molecular Weight 171 kDa
GenBank Accession NumberBC054511
Gene Symbol DLC1
Gene ID (NCBI) 10395
RRIDAB_2246077
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QB1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for DLC1 antibody 15460-1-APDownload protocol
IF protocol for DLC1 antibody 15460-1-APDownload protocol
IP protocol for DLC1 antibody 15460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biomaterials

Stiffness assisted cell-matrix remodeling trigger 3D mechanotransduction regulatory programs

Authors - Anna L Kersey
View Article