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  • Featured Product
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DLEU1 Polyclonal antibody

DLEU1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11860-1-AP

Print datasheet

Synonyms

DLB1, DLEU1, DLEU2, LEU1, LEU2, NCRNA00021, XTP6

验证数据展示

  • IHC staining of human colon cancer using 11860-1-AP

    IHC staining of human colon cancer using 11860-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11860-1-AP (DLEU1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of K-562 using 11860-1-AP

    WB analysis of K-562 using 11860-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11860-1-AP (DLEU1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 11860-1-AP

    WB analysis of A549 using 11860-1-AP

    WB result of DLEU1 antibody (11860-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLEU1 transfected A549 cells.

经过测试的应用

Positive WB detected inK-562 cells, A549 cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11860-1-AP targets DLEU1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen DLEU1 fusion protein Ag2435 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deleted in lymphocytic leukemia 1 (non-protein coding)
Synonyms DLB1, DLEU1, DLEU2, LEU1, LEU2, NCRNA00021, XTP6
Calculated Molecular Weight 78 aa, 9 kDa
Observed Molecular Weight 27-30 kDa
GenBank Accession NumberBC020692
Gene Symbol DLEU1
Gene ID (NCBI) 10301
RRIDAB_874117
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43261
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DLEU1 antibody 11860-1-APDownload protocol
IHC protocol for DLEU1 antibody 11860-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncotarget

The effects of DLEU1 gene expression in Burkitt lymphoma (BL): potential mechanism of chemoimmunotherapy resistance in BL.

Authors - Sanghoon Lee
View Article