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SAP102 Polyclonal antibody

SAP102 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18036-1-AP

Print datasheet

Synonyms

Disks large homolog 3, DLG3, KIAA1232, MRX, MRX90, NE Dlg, NEDLG, Neuroendocrine DLG, SAP 102, SAP102, Synapse associated protein 102, XLMR

验证数据展示

  • WB analysis using 18036-1-AP

    WB analysis using 18036-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18036-1-AP (SAP102 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 18036-1-AP

    IP experiment of mouse brain using 18036-1-AP

    IP result of anti-SAP102 (IP:18036-1-AP, 3ug; Detection:18036-1-AP 1:3000) with mouse brain tissue lysate 4000ug.

  • IHC staining of mouse brain using 18036-1-AP

    IHC staining of mouse brain using 18036-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18036-1-AP (SAP102 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18036-1-AP targets SAP102 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen SAP102 fusion protein Ag12658 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name discs, large homolog 3 (Drosophila)
Synonyms Disks large homolog 3, DLG3, KIAA1232, MRX, MRX90, NE Dlg, NEDLG, Neuroendocrine DLG, SAP 102, SAP102, Synapse associated protein 102, XLMR
Calculated Molecular Weight 817 aa, 90 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC093864
Gene Symbol SAP102
Gene ID (NCBI) 1741
RRIDAB_2092175
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92796
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synapse-associated protein 102 (SAP102), also known as DLG3, is a MAGUK that is highly expressed early in development and mediates receptor trafficking during synaptogenesis. The importance of SAP102 function in brain development is demonstrated by the fact that SAP102 mutations have been identified in human patients with nonsyndromic X-linked mental retardation (XLMR).SAP102 has critical roles in early cortical synapse development.

实验方案

Product Specific Protocols
WB protocol for SAP102 antibody 18036-1-APDownload protocol
IHC protocol for SAP102 antibody 18036-1-APDownload protocol
IP protocol for SAP102 antibody 18036-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Clin Exp Pharmacol Physiol

High expression of DLG3 is associated with decreased survival from breast cancer.

Authors - Jie Liu
View Article
mouseWB

J Nutr Biochem

Hippocampal proteomic changes in high-fat diet-induced obese mice associated with memory decline

Authors - Ping Lu
View Article