• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

DLG5 Polyclonal antibody

DLG5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15687-1-AP

Print datasheet

Synonyms

Placenta and prostate DLG, PDLG, KIAA0583, Disks large homolog 5, Discs large protein P-dlg

验证数据展示

  • WB analysis of PC-3 using 15687-1-AP

    WB analysis of PC-3 using 15687-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15687-1-AP (DLG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 15687-1-AP

    WB analysis using 15687-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15687-1-AP (DLG5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of Human bowens disease using 15687-1-AP

    IHC staining of Human bowens disease using 15687-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 15687-1-AP (DLG5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of Human bowens disease using 15687-1-AP

    IHC staining of Human bowens disease using 15687-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 15687-1-AP (DLG5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inPC-3 cells, mouse kidney tissue, HeLa cells
Positive IHC detected inHuman bowens disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15687-1-AP targets DLG5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen DLG5 fusion protein Ag8194 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name discs, large homolog 5 (Drosophila)
Synonyms Placenta and prostate DLG, PDLG, KIAA0583, Disks large homolog 5, Discs large protein P-dlg
Calculated Molecular Weight 1893 aa, 209 kDa
Observed Molecular Weight 75 kDa, 240 kDa
GenBank Accession NumberBC002915
Gene Symbol DLG5
Gene ID (NCBI) 9231
RRIDAB_10642568
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDM6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Discs large homolog 5 (DLG5) belongs to the membrane-associated guanylate kinase (MAGUK) family. DLG5 participates in epithelial cell polarity maintenance and cancer development. DLG5 is highly expressed in normal tissues, but its expression is decreased in many cancers, such as bladder cancer, prostate cancer, breast cancer, and hepatocellular carcinoma (PMID: 32015336). Down-regulation of DLG5 is highly correlated with clinical tumor stage. In breast cancer, knockdown of DLG5 induces cell migration, and overexpression of DLG5 inhibits cell migration (PMID: 28169360). DLG5 has 3 isoforms with the molecular weight of 75, 214 and 240 kDa.

实验方案

Product Specific Protocols
WB protocol for DLG5 antibody 15687-1-APDownload protocol
IHC protocol for DLG5 antibody 15687-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

PABPC1-induced stabilization of BDNF-AS inhibits malignant progression of glioblastoma cells through STAU1-mediated decay.

Authors - Rui Su
View Article