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  • Featured Product
  • KD/KO Validated

DLGAP3 Polyclonal antibody

DLGAP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 55056-1-AP

Print datasheet

Synonyms

PSD-95/SAP90-binding protein 3, PSD 95/SAP90 binding protein 3, Disks large-associated protein 3, DAP-3, DAP3

验证数据展示

  • WB analysis of human brain using 55056-1-AP

    WB analysis of human brain using 55056-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55056-1-AP (DLGAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 55056-1-AP

    IHC staining of human stomach using 55056-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55056-1-AP (DLGAP3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman brain tissue, mouse brain tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55056-1-AP targets DLGAP3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name discs, large (Drosophila) homolog-associated protein 3
Synonyms PSD-95/SAP90-binding protein 3, PSD 95/SAP90 binding protein 3, Disks large-associated protein 3, DAP-3, DAP3
Calculated Molecular Weight 106 kDa
Observed Molecular Weight 106-120 kDa
GenBank Accession NumberNM_001080418
Gene Symbol DLGAP3
Gene ID (NCBI) 58512
RRIDAB_10858793
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95886
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DLGAP3, also named as DAP3, belongs to the SAPAP family. It may play a role in the molecular organization of synapses and neuronal cell signaling. DLGAP3 could be an adapter protein linking ion channel to the subsynaptic cytoskeleton. It may induce enrichment of PSD-95/SAP90 at the plasma membrane. The antibody has no cross reaction to other DLGAP members.

实验方案

Product Specific Protocols
WB protocol for DLGAP3 antibody 55056-1-APDownload protocol
IHC protocol for DLGAP3 antibody 55056-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Biol Psychiatry

Metabotropic Glutamate Receptor and Fragile X Signaling in a Female Model of Escalated Aggression.

Authors - Laura E Been
View Article
human,mouseWB,IF

Cell Death Dis

SAPAP3 regulates epileptic seizures involving GluN2A in post-synaptic densities.

Authors - Yanke Zhang
  • KD Validated
View Article
mouseWB

Nat Neurosci

Neuronal activity rapidly reprograms dendritic translation via eIF4G2:uORF binding

Authors - Ezgi Hacisuleyman
View Article