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DLK1 Polyclonal antibody, PBS Only

DLK1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29288-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 29288-1-AP (same clone as 29288-1-PBS)

    WB analysis using 29288-1-AP (same clone as 29288-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29288-1-AP (DLK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29288-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 29288-1-AP (same clone as 29288-1-PBS)

    IHC staining of human placenta using 29288-1-AP (same clone as 29288-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29288-1-AP (DLK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29288-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DLK1 antibody included
  • IHC staining of human placenta using 29288-1-AP (same clone as 29288-1-PBS)

    IHC staining of human placenta using 29288-1-AP (same clone as 29288-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29288-1-AP (DLK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29288-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DLK1 antibody included
Planning an IHC experiment? We recommend our IHCeasy DLK1 Ready-To-Use IHC Kit. DLK1 primary antibody included.

产品信息

29288-1-PBS targets DLK1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DLK1 fusion protein Ag30916 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name delta-like 1 homolog (Drosophila)
Synonyms
Calculated Molecular Weight 41 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC007741
Gene Symbol DLK1
Gene ID (NCBI) 8788
RRIDAB_2918271
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP80370
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DLK1, also named PREF1, FA1, or pG2, is a transmembrane protein belonging to the epidermal growth factor (EGF)-like superfamily (PMID: 8095043). It contains six EGF-like repeats in the extracellular region. DLK1 is abundant in preadipocytes and regulate adipocyte differentiation negatively (PMID: 8500166). Deficiency of DLK1 gives rise to growth retardation and accelerated adiposity in mouse model. Expression of DLK1 is found in tumors with neuroendocrine features that implies DLK1 may be involved in neuroendocrine differentiation (PMID: 8095043). It has been reported overexpression of DLK1 could lead to the development of metabolic abnormalities by impairment of adipocyte function in mice (PMID: 12588883). The gene of DLK1 maps to chromosome 14q32, and encodes a 383-amino acid protein with a calculated molecular mass of 41 kDa. In preadipocytes, multiple discrete forms of DLK1 protein of 45-60 kDa are present, owing in part to N-linked glycosylation (PMID: 8500166).