DLK1 Monoclonal antibody

DLK1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67923-1-Ig

产品说明书

CloneNo. 2C3H8

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IHC, ELISA

pG2, FA1, DLK-1, DLK 1, DLK

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJAR cells, human placenta tissue, pig adrenal gland tissue, NCCIT cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DLK1 Ready-To-Use IHC Kit. DLK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67923-1-Ig targets DLK1 in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, pig
免疫原 DLK1 fusion protein Ag30916 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 delta-like 1 homolog (Drosophila)
别名 pG2, FA1, DLK-1, DLK 1, DLK
计算分子量 41 kDa
观测分子量45-60 kDa
GenBank蛋白编号BC007741
基因名称 DLK1
Gene ID (NCBI) 8788
RRIDAB_2918675
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP80370
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DLK1, also named PREF1, FA1, or pG2, is a transmembrane protein belonging to the epidermal growth factor (EGF)-like superfamily (PMID: 8095043). It contains six EGF-like repeats in the extracellular region. DLK1 is abundant in preadipocytes and regulate adipocyte differentiation negatively (PMID: 8500166). Deficiency of DLK1 gives rise to growth retardation and accelerated adiposity in mouse model. Expression of DLK1 is found in tumors with neuroendocrine features that implies DLK1 may be involved in neuroendocrine differentiation (PMID: 8095043). It has been reported overexpression of DLK1 could lead to the development of metabolic abnormalities by impairment of adipocyte function in mice (PMID: 12588883). The gene of DLK1 maps to chromosome 14q32, and encodes a 383-amino acid protein with a calculated molecular mass of 41 kDa. In preadipocytes, multiple discrete forms of DLK1 protein of 45-60 kDa are present, owing in part to N-linked glycosylation (PMID: 8500166).

实验方案

Product Specific Protocols
WB protocol for DLK1 antibody 67923-1-IgDownload protocol
IHC protocol for DLK1 antibody 67923-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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