验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue, MG-63 cells, HeLa cells, HUVEC cells, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
28544-1-AP targets DLL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | DLL1 fusion protein Ag29534 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | delta-like 1 (Drosophila) |
| 别名 | H-Delta-1, Drosophila Delta homolog 1, Delta-like protein 1, Delta1, Delta like protein 1 |
| 计算分子量 | 78 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | NM_005618 |
| 基因名称 | DLL1 |
| Gene ID (NCBI) | 28514 |
| RRID | AB_3086063 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00548 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Notch pathway functions during diverse developmental and physiological processes. Notch receptor and its ligands, Delta and Jagged, are transmembrane proteins with large extracellular domains that consist primarily of epidermal growth factor (EGF)-like repeats (PMID: 16921404). Delta-like protein 1 (DLL1, also named as Delta1) is a ligand of NOTCH1, NOTCH2 and NOTCH3 receptors that binds the extracellular domain of Notch receptor in a cis and trans fashion manner (PMID: 11006133). DLL1 play a role in development of the central nervous system, somites and lymphocytes (PMID: 31353024). It is overexpressed in various cancers and is involved in the regulation of cancer progression (PMID: 32440154). DLL1 undergoes proteolytic processing in its extracellular domain by ADAM proteases and gamma-secretase (PMID: 17107962; 12794186).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DLL1 antibody 28544-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Phytomedicine Celastrol ameliorates fibrosis in Western diet/tetrachloromethane-induced nonalcoholic steatohepatitis by suppressing Notch/osteopontin signaling | ||
Drug Deliv Transl Res LINC00460 promotes neuroblastoma tumorigenesis and cisplatin resistance by targeting miR-149-5p/DLL1 axis and activating Notch pathway in vitro and in vivo |
