DLL1 Polyclonal antibody
DLL1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue, MG-63 cells, HeLa cells, HUVEC cells, mouse brain tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
28544-1-AP targets DLL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | DLL1 fusion protein Ag29534 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | delta-like 1 (Drosophila) |
| Synonyms | H-Delta-1, Drosophila Delta homolog 1, Delta-like protein 1, Delta1, Delta like protein 1 |
| Calculated Molecular Weight | 78 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | NM_005618 |
| Gene Symbol | DLL1 |
| Gene ID (NCBI) | 28514 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00548 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Notch pathway functions during diverse developmental and physiological processes. Notch receptor and its ligands, Delta and Jagged, are transmembrane proteins with large extracellular domains that consist primarily of epidermal growth factor (EGF)-like repeats (PMID: 16921404). Delta-like protein 1 (DLL1, also named as Delta1) is a ligand of NOTCH1, NOTCH2 and NOTCH3 receptors that binds the extracellular domain of Notch receptor in a cis and trans fashion manner (PMID: 11006133). DLL1 play a role in development of the central nervous system, somites and lymphocytes (PMID: 31353024). It is overexpressed in various cancers and is involved in the regulation of cancer progression (PMID: 32440154). DLL1 undergoes proteolytic processing in its extracellular domain by ADAM proteases and gamma-secretase (PMID: 17107962; 12794186).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DLL1 antibody 28544-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Drug Deliv Transl Res LINC00460 promotes neuroblastoma tumorigenesis and cisplatin resistance by targeting miR-149-5p/DLL1 axis and activating Notch pathway in vitro and in vivo |
