验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, A375 cells, U-251 cells |
| Positive IHC detected in | human thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 3 publications below |
产品信息
10592-1-AP targets DLX5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | DLX5 fusion protein Ag0910 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | distal-less homeobox 5 |
| 别名 | distal less homeobox 5, DLX5, Homeobox protein DLX 5 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC006226 |
| 基因名称 | DLX5 |
| Gene ID (NCBI) | 1749 |
| RRID | AB_2090724 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P56178 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DLX5 belongs to the DLX family of homeobox transcription factors (Dlx1, Dlx2, Dlx5 and Dlx6), which is expressed in overlapping domains at different stages of cell differentiation in the subpallium and controls differentiation of GABAergic neurons. DLX5 is an immediate early BMP-responsive transcriptional activator that essential for osteoblast differentiation. It can bind to and stimulate the homeodomain-response element of the ALPL and SP7 promoter. DLX5 can form homodimer with DLX2(~34kd) and heterodimer with MSX2(~29kd).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DLX5 antibody 10592-1-AP | Download protocol |
| IHC protocol for DLX5 antibody 10592-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Activation of bivalent factor DLX5 cooperates with master regulator TP63 to promote squamous cell carcinoma.
| ||
Acta Biomater Osteoinductivity and biomechanical assessment of a 3D printed demineralized bone matrix-ceramic composite in a rat spine fusion model. | ||
J Cell Physiol Long noncoding RNA FBXL19-AS1 induces tumor growth and metastasis by sponging miR-203a-3p in lung adenocarcinoma. | ||
Arthritis Res Ther Ossification process involving the human thoracic ligamentum flavum: role of transcription factors. | ||
PLoS One Involvement of microRNAs in regulation of osteoblastic differentiation in mouse induced pluripotent stem cells. | ||
J Oral Maxillofac Surg Zoledronic Acid-Treated Rats Show Altered Jaw Histology and Gene Expression in Nonexposed Medication-Related Osteonecrosis of the Jaws |
