验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HeLa cells, NIH/3T3 cells |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10411-1-AP targets DMAP1 in WB, IHC, IP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB, ChIP, RIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | DMAP1 fusion protein Ag0665 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | DNA methyltransferase 1 associated protein 1 |
| Synonyms | EAF2, DNMTAP1, DNMT1-associated protein 1, DNMT1 associated protein 1, DNMAP1 |
| Calculated Molecular Weight | 53 kDa |
| Observed Molecular Weight | 53-60 kDa |
| GenBank Accession Number | BC002855 |
| Gene Symbol | DMAP1 |
| Gene ID (NCBI) | 55929 |
| RRID | AB_2277167 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NPF5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DMAP1 (DNA methyltransferase 1-associated protein 1) a component of the NuA4 histone acetyltransferase complex, was originally identified as an interacting molecule with DNA methyltransferase 1 (DNMT1) which co-localizes with PCNA at DNA replication foci. DMAP1 is required for DNA repair and acts against genomic instability. DMAP1 is widely implicated in transcription repression and activation via its interaction with multiple targets including histone deacetylase2 (HDAC2). The antibody reacts specifically with DMAP1 protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DMAP1 antibody 10411-1-AP | Download protocol |
| IHC protocol for DMAP1 antibody 10411-1-AP | Download protocol |
| IP protocol for DMAP1 antibody 10411-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell An RNAi screen of chromatin proteins identifies Tip60-p400 as a regulator of embryonic stem cell identity.
| ||
Nat Struct Mol Biol R loops regulate promoter-proximal chromatin architecture and cellular differentiation. | ||
EMBO Mol Med Phosphorylation of TFCP2L1 by CDK1 is required for stem cell pluripotency and bladder carcinogenesis. | ||
Cell Rep Sirt1 Regulates DNA Methylation and Differentiation Potential of Embryonic Stem Cells by Antagonizing Dnmt3l. | ||
Psychiatry Investig m6A Methyltransferase METTL3 Reduces Hippocampal Neuron Apoptosis in a Mouse Model of Autism Through the MALAT1/SFRP2/Wnt/β-catenin Axis |
