DMC1 Polyclonal antibody

DMC1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13714-1-AP

Print datasheet

Synonyms

LIM15, HsLim15, DMC1H



经过测试的应用

Positive WB detected inmouse testis tissue, HeLa cells, human testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:600-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13714-1-AP targets DMC1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen DMC1 fusion protein Ag4653 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast)
Synonyms LIM15, HsLim15, DMC1H
Calculated Molecular Weight 165 aa, 18 kDa, 38 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC035658
Gene Symbol DMC1
Gene ID (NCBI) 11144
RRIDAB_2091076
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14565
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DMC1 antibody 13714-1-APDownload protocol
IHC protocol for DMC1 antibody 13714-1-APDownload protocol
IF protocol for DMC1 antibody 13714-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nucleic Acids Res

NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells.

Authors - Lu Chen
mouseIF

Nucleic Acids Res

MEIOK21: a new component of meiotic recombination bridges required for spermatogenesis.

Authors - Yongliang Shang

Cell Prolif

ZFP541 and KCTD19 regulate chromatin organization and transcription programs for male meiotic progression

Authors - Xu Zhou
mouseIF

Cell Death Differ

NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis.

Authors - Bin Zhang
mouseIF

Elife

A missense in HSF2BP causing primary ovarian insufficiency affects meiotic recombination by its novel interactor C19ORF57/BRME1.

Authors - Natalia Felipe-Medina
humanIHC

Front Oncol

The Ectopic Expression of Meiosis Regulatory Genes in Cutaneous T-Cell Lymphomas (CTCL).

Authors - Jennifer Gantchev