DMC1 Polyclonal antibody
DMC1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IF-P, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse testis tissue, HeLa cells, human testis tissue, rat testis tissue |
Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse testis tissue |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Immunofluorescence (IF)-P | IF-P : 1:600-1:2400 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13714-1-AP targets DMC1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | DMC1 fusion protein Ag4653 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) |
Synonyms | LIM15, HsLim15, DMC1H |
Calculated Molecular Weight | 165 aa, 18 kDa, 38 kDa |
Observed Molecular Weight | 37 kDa |
GenBank Accession Number | BC035658 |
Gene Symbol | DMC1 |
Gene ID (NCBI) | 11144 |
RRID | AB_2091076 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q14565 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for DMC1 antibody 13714-1-AP | Download protocol |
IHC protocol for DMC1 antibody 13714-1-AP | Download protocol |
IF protocol for DMC1 antibody 13714-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nucleic Acids Res NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells. | ||
Nucleic Acids Res MEIOK21: a new component of meiotic recombination bridges required for spermatogenesis. | ||
Cell Prolif ZFP541 and KCTD19 regulate chromatin organization and transcription programs for male meiotic progression | ||
Cell Death Differ NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis. | ||
Elife A missense in HSF2BP causing primary ovarian insufficiency affects meiotic recombination by its novel interactor C19ORF57/BRME1. | ||
Front Oncol The Ectopic Expression of Meiosis Regulatory Genes in Cutaneous T-Cell Lymphomas (CTCL). |