DMC1 Monoclonal antibody
DMC1 Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, IP, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2D5C9
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, human testis tissue, rat testis, mouse testis |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
67176-1-Ig targets DMC1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF-P, IP, ELISA Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | DMC1 fusion protein Ag6951 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) |
| Synonyms | LIM15, HsLim15, DMC1H, 2D5C9 |
| Calculated Molecular Weight | 165 aa, 18 kDa, 38 kDa |
| Observed Molecular Weight | 37 kDa |
| GenBank Accession Number | BC035658 |
| Gene Symbol | DMC1 |
| Gene ID (NCBI) | 11144 |
| RRID | AB_2882472 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14565 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DMC1 antibody 67176-1-Ig | Download protocol |
| IHC protocol for DMC1 antibody 67176-1-Ig | Download protocol |
| IF protocol for DMC1 antibody 67176-1-Ig | Download protocol |
| IP protocol for DMC1 antibody 67176-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
