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DMC1 Monoclonal antibody

DMC1 Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2D5C9

Cat no : 67176-1-Ig

Print datasheet

Synonyms

LIM15, HsLim15, DMC1H, 2D5C9

验证数据展示

  • WB analysis of HeLa using 67176-1-Ig

    WB analysis of HeLa using 67176-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67176-1-Ig (DMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 67176-1-Ig

    IHC staining of mouse testis using 67176-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67176-1-Ig (DMC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 67176-1-Ig

    IF Staining of mouse testis using 67176-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DMC1 antibody (67176-1-Ig, Clone: 2D5C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue).

  • IF Staining of mouse testis using 67176-1-Ig

    IF Staining of mouse testis using 67176-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DMC1 antibody (67176-1-Ig, Clone: 2D5C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue).

  • IP experiment of mouse testis using 67176-1-Ig

    IP experiment of mouse testis using 67176-1-Ig

    IP result of anti-DMC1 (IP:67176-1-Ig, 4ug; Detection:67176-1-Ig 1:1000) with mouse testis tissue lysate 2040 ug.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, human testis tissue, rat testis, mouse testis
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

67176-1-Ig targets DMC1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, IP, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen DMC1 fusion protein Ag6951 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast)
Synonyms LIM15, HsLim15, DMC1H, 2D5C9
Calculated Molecular Weight 165 aa, 18 kDa, 38 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC035658
Gene Symbol DMC1
Gene ID (NCBI) 11144
RRIDAB_2882472
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14565
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DMC1 antibody 67176-1-IgDownload protocol
IHC protocol for DMC1 antibody 67176-1-IgDownload protocol
IF protocol for DMC1 antibody 67176-1-IgDownload protocol
IP protocol for DMC1 antibody 67176-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

J Genet Genomics

The novel male meiosis recombination regulator coordinates the progression of meiosis prophase I.

Authors - Miao Li
View Article