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验证数据展示

  • WB analysis of HeLa using 67176-1-Ig

    WB analysis of HeLa using 67176-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67176-1-Ig (DMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 67176-1-Ig

    IHC staining of mouse testis using 67176-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67176-1-Ig (DMC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DMC1 antibody included
  • IF Staining of mouse testis using 67176-1-Ig

    IF Staining of mouse testis using 67176-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DMC1 antibody (67176-1-Ig, Clone: 2D5C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue).

  • IF Staining of mouse testis using 67176-1-Ig

    IF Staining of mouse testis using 67176-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DMC1 antibody (67176-1-Ig, Clone: 2D5C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue).

  • IP experiment of mouse testis using 67176-1-Ig

    IP experiment of mouse testis using 67176-1-Ig

    IP result of anti-DMC1 (IP:67176-1-Ig, 4ug; Detection:67176-1-Ig 1:1000) with mouse testis tissue lysate 2040 ug.

DMC1 Monoclonal antibody

DMC1 Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 67176-1-Ig

产品说明书

CloneNo. 2D5C9
引用文献(2)

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, ELISA and More (1)

别名

LIM15, HsLim15, DMC1H, 2D5C9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, human testis tissue, rat testis, mouse testis
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Planning an IHC experiment? We recommend our IHCeasy DMC1 Ready-To-Use IHC Kit. DMC1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 2 publications below

产品信息

67176-1-Ig targets DMC1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 DMC1 fusion protein Ag6951 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast)
别名 LIM15, HsLim15, DMC1H, 2D5C9
计算分子量 165 aa, 18 kDa, 38 kDa
观测分子量 37 kDa
GenBank蛋白编号BC035658
基因名称 DMC1
Gene ID (NCBI) 11144
RRIDAB_2882472
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14565
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DMC1 antibody 67176-1-IgDownload protocol
IHC protocol for DMC1 antibody 67176-1-IgDownload protocol
IF protocol for DMC1 antibody 67176-1-IgDownload protocol
IP protocol for DMC1 antibody 67176-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

J Genet Genomics

The novel male meiosis recombination regulator coordinates the progression of meiosis prophase I.

Authors - Miao Li
View Article
mouseIF

Elife

ARID1A governs the silencing of sex-linked transcription during male meiosis in the mouse

Authors - Debashish U Menon
View Article